Phenotypes associated with this allele

• Allele Symbol: Amigo1^{tm1.1(KOMP)Vlcg}
• Allele Name: targeted mutation 1.1, Velocigene
• Allele ID: MGI:5634962
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Amigo1^tm1.1(KOMP)Vlcg/Amigo1^tm1.1(KOMP)Vlcg	B6N(Cg)-Amigo1^tm1.1(KOMP)Vlcg/2J	MGI:6261854
hm2	Amigo1^tm1.1(KOMP)Vlcg/Amigo1^tm1.1(KOMP)Vlcg	involves: C57BL/6N	MGI:7449207
cx3	Amigo1^tm1.1(KOMP)Vlcg/Amigo1^tm1.1(KOMP)Vlcg Tg(Gad1-EGFP)G42Zjh/0	involves: C57BL/6 * C57BL/6N	MGI:7449209
cx4	Amigo1^tm1.1(KOMP)Vlcg/Amigo1^tm1.1(KOMP)Vlcg Amigo2^em#Dke/Amigo2^em#Dke	involves: C57BL/6J * C57BL/6N * DBA/2	MGI:7449215

Genotype
MGI:6261854

hm1

• Allelic Composition: Amigo1^{tm1.1(KOMP)Vlcg}/Amigo1^{tm1.1(KOMP)Vlcg}
• Genetic Background: B6N(Cg)-Amigo1^{tm1.1(KOMP)Vlcg}/2J
• Cell Lines: 10669A-H9

Data Sources

IMPC Data for Amigo1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

hyperactivity ( J:211773 )

IMPC - JAX

increased vertical activity ( J:211773 )

♀

IMPC - JAX

Genotype
MGI:7449207

hm2

• Allelic Composition: Amigo1^{tm1.1(KOMP)Vlcg}/Amigo1^{tm1.1(KOMP)Vlcg}
• Genetic Background: involves: C57BL/6N
• Cell Lines: 10669A-E11

vision/eye

vision/eye phenotype ( J:333740 )

N • at P30, the average density and density recovery profiles of retinal horizontal cells is normal, indicating that horizontal cell mosaics are preserved

N • in vivo electroretinographs showed that dark-adapted a- and b-wave amplitudes (reflecting rod and rod bipolar cell responses, respectively) as well as light-adapted b-wave amplitudes (reflecting cone bipolar cell responses to bright stimuli) are normal

N • ganglion cell spike trains elicited by dim and bright light stimuli (i.e., the retinal output) are also normal

abnormal retina rod bipolar cell morphology ( J:333740 )

• after intravitreal injections of AAV-Grm6-tdTomato, rod bipolar cell dendrites are significantly smaller than in wild-type controls, indicating that changes in horizontal cell axons are matched by changes in synaptic partner dendrites to stabilize retinal function

• however, the density of their connections with rods is normal and rod bipolar cell axon size is unaffected

abnormal retina horizontal cell morphology ( J:333740 )

• when horizontal cells are sparsely labeled by AAV-CAG-YFP, horizontal cell axons are significantly smaller than in wild-type controls

• however, the densities of horizontal cell axon tips and dendrite clusters as well as the density of axon branches are unaffected

nervous system

abnormal retina rod bipolar cell morphology ( J:333740 )

• after intravitreal injections of AAV-Grm6-tdTomato, rod bipolar cell dendrites are significantly smaller than in wild-type controls, indicating that changes in horizontal cell axons are matched by changes in synaptic partner dendrites to stabilize retinal function

• however, the density of their connections with rods is normal and rod bipolar cell axon size is unaffected

abnormal retina horizontal cell morphology ( J:333740 )

• when horizontal cells are sparsely labeled by AAV-CAG-YFP, horizontal cell axons are significantly smaller than in wild-type controls

• however, the densities of horizontal cell axon tips and dendrite clusters as well as the density of axon branches are unaffected

Genotype
MGI:7449209

cx3

• Allelic Composition: Amigo1^{tm1.1(KOMP)Vlcg}/Amigo1^{tm1.1(KOMP)Vlcg}; Tg(Gad1-EGFP)G42Zjh/0
• Genetic Background: involves: C57BL/6 * C57BL/6N
• Cell Lines: 10669A-E11

vision/eye

abnormal retina horizontal cell morphology ( J:333740 )

• retinal vibratome slices stained for neurofilament show that developing horizontal cell axons are mistargeted into the outer nuclear layer from P10

• however, rod bipolar cell dendrites do not make the same targeting errors as horizontal cell axons and the stratification patterns of other neurons and ribbon synapses are normal

nervous system

abnormal retina horizontal cell morphology ( J:333740 )

• retinal vibratome slices stained for neurofilament show that developing horizontal cell axons are mistargeted into the outer nuclear layer from P10

• however, rod bipolar cell dendrites do not make the same targeting errors as horizontal cell axons and the stratification patterns of other neurons and ribbon synapses are normal

Genotype
MGI:7449215

cx4

• Allelic Composition: Amigo1^{tm1.1(KOMP)Vlcg}/Amigo1^{tm1.1(KOMP)Vlcg}; Amigo2^em#Dke/Amigo2^em#Dke
• Genetic Background: involves: C57BL/6J * C57BL/6N * DBA/2
• Cell Lines: 10669A-E11

vision/eye

vision/eye phenotype ( J:333740 )

N • dark-adapted a- and b-wave amplitudes and light-adapted b-wave amplitudes are not significantly different from those in wild-type controls, indicating normal ERG responses