Phenotypes associated with this allele

• Allele Symbol: Rxfp4^tm1Sora
• Allele Name: targeted mutation 1, S O'Rahilly
• Allele ID: MGI:5637423
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rxfp4^tm1Sora/Rxfp4^tm1Sora	129S/SvEv-Rxfp4^tm1Sora	MGI:5662484

Genotype
MGI:5662484

hm1

• Allelic Composition: Rxfp4^tm1Sora/Rxfp4^tm1Sora
• Genetic Background: 129S/SvEv-Rxfp4^tm1Sora

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:212239 )

• at 3 months of age, mutant mice tend to have a lower body weight than wild-type controls

• however, when fed on HFD or HCD for 22 weeks, mutant mice eventually reach similar body weights to wild-type controls

behavior/neurological

behavior/neurological phenotype ( J:212239 )

N • mutant mice are viable and fertile and show no detectable differences in a number of morphological, neurological, behavioral assays relative to wild-type controls

abnormal food intake ( J:212239 )

• i.p. injection of insulin-like peptide 5 (Insl5), a newly identified orexigenic gastrointestinal hormone, at a concentration of 1000 ng/25g, fails to increase food intake in nonfasted mutant mice, unlike in similarly-treated wild-type controls

• i.v. injection of anti-Insl5 antibody in overnight-fasted mutant mice fails to reduce food intake over the following 24-h period, unlike in similarly-treated wild-type controls

decreased compensatory feeding amount ( J:212239 )

• overnight-fasted mutant mice presented with a choice of a HFD (high-fat diet) and a high-carbohydrate diet (HCD) exhibit shorter meal durations than wild-type controls; this is particularly pronounced for HFD

abnormal food preference ( J:212239 )

• overnight-fasted mutant mice presented with a choice of a HFD and a HCD, offered in parallel, appear to lose the preference for HFD seen in wild-type controls

• when fasted overnight before being offered only a HFD for the first 24 hrs, and then an additional choice of HCD for a further 2 days, mutant mice consume less HFD than wild-type controls throughout the 3-day observation period

• when additionally offered HCD, the cumulative combined intake of both diets remains lower in mutant mice, although they eventually consume more HCD than wild-type controls

adipose tissue

decreased total body fat amount ( J:212239 )

• at 11 weeks of age, mutant mice fed on a chow diet exhibit a significantly reduced fat content relative to wild-type controls, as shown by NMR spectroscopy

• however, no significant differences in body weight or lean mass are observed

decreased gonadal fat pad weight ( J:212239 )

• ex vivo, 11-week-old mutant mice fed on a chow diet have significantly smaller gonadal fat pad masses than wild-type controls

decreased inguinal fat pad weight ( J:212239 )

• ex vivo, 11-week-old mutant mice fed on a chow diet have significantly smaller inguinal fat pad masses than wild-type controls

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:212239 )

N • mutant mice exhibit no detectable differences in clinical chemistry, hematology or glucose metabolism relative to wild-type controls