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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Rnase9tm1.1Xen
targeted mutation 1.1, Taconic Biosciences
MGI:5637811
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Rnase9tm1.1Xen/Rnase9tm1.1Xen involves: 129S6/SvEvTac MGI:5767002
ht2
 		Rnase9tm1.1Xen/Rnase9+ involves: 129S6/SvEvTac MGI:5767001


Genotype
MGI:5767002
hm1
Allelic
Composition		 Rnase9tm1.1Xen/Rnase9tm1.1Xen
Genetic
Background		 involves: 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rnase9tm1.1Xen mutation (1 available); any Rnase9 mutation (19 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:212760 )
N
• homozygotes of both sexes are fertile in unrestricted mating trials
• sexually mature males show normal testis and epididymis weight and histology as well as normal sperm morphology, indicating normal spermatogenesis
• in vitro fertilization assays revealed normal fertilization rates between wild-type and mutant sperm
abnormal sperm motility ( J:212760 )
• visual analysis of swim out drops during isolation of mutant corpus sperm showed decreased cloudiness relative to wild-type controls, indicating slower or delayed release of sperm from the epididymis
• analysis of sperm velocities shortly after release (~1 min) from the corpus revealed that straight line velocity (VSL) of mutant sperm is significantly lower than in wild-type sperm
• however, no differences are detected by computer-assisted sperm analysis 10-90 min after sperm isolation from the corpus or cauda, indicating that impairment in corpus sperm motility is transient and normalizes within 10 min after release of sperm from the epididymis
impaired sperm capacitation ( J:212760 )
• mutant caudal sperm display decreased phosphorylation of protein kinase A (PKA) substrates upon capacitation
• in contrast, levels of tyrosine phosphorylation of sperm proteins are normal

cellular
abnormal sperm motility ( J:212760 )
• visual analysis of swim out drops during isolation of mutant corpus sperm showed decreased cloudiness relative to wild-type controls, indicating slower or delayed release of sperm from the epididymis
• analysis of sperm velocities shortly after release (~1 min) from the corpus revealed that straight line velocity (VSL) of mutant sperm is significantly lower than in wild-type sperm
• however, no differences are detected by computer-assisted sperm analysis 10-90 min after sperm isolation from the corpus or cauda, indicating that impairment in corpus sperm motility is transient and normalizes within 10 min after release of sperm from the epididymis




Genotype
MGI:5767001
ht2
Allelic
Composition		 Rnase9tm1.1Xen/Rnase9+
Genetic
Background		 involves: 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rnase9tm1.1Xen mutation (1 available); any Rnase9 mutation (19 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:212760 )
• heterozygotes of both sexes are overtly normal and show normal reproductive performance




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory