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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Slc12a5tm2Hsav
targeted mutation 2, Harri Savilahti
MGI:5638435
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Slc12a5tm2Hsav/Slc12a5tm2Hsav involves: 129S1/Sv * 129X1/SvJ * C57BL/6J MGI:5638536
ht2
Slc12a5tm1Hsav/Slc12a5tm2Hsav involves: 129S1/Sv * 129S2/SvPasHsd * 129X1/SvJ * C57BL/6J * C57BL/6JOlaHsd MGI:5638539
ht3
Slc12a5tm1Hsav/Slc12a5tm2Hsav involves: 129S1/Sv * 129X1/SvJ * C57BL/6J MGI:5638537


Genotype
MGI:5638536
hm1
Allelic
Composition
Slc12a5tm2Hsav/Slc12a5tm2Hsav
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc12a5tm2Hsav mutation (0 available); any Slc12a5 mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• primary cultures of cortical neurons show longer dendritic protrusions of pyramidal-shaped neurons than in wild-type cultures, with varied morphology from filopodia-like structures to protrusions with clear bulbous heads
• the number of functional dendritic synapses is lower in neurons
• lower frequency of miniature excitatory postsynaptic currents (mEPSCs) in neurons but no difference in amplitude




Genotype
MGI:5638539
ht2
Allelic
Composition
Slc12a5tm1Hsav/Slc12a5tm2Hsav
Genetic
Background
involves: 129S1/Sv * 129S2/SvPasHsd * 129X1/SvJ * C57BL/6J * C57BL/6JOlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc12a5tm1Hsav mutation (1 available); any Slc12a5 mutation (45 available)
Slc12a5tm2Hsav mutation (0 available); any Slc12a5 mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• mice exhibit normal weight at birth, however, by 2 weeks of age, they weigh about 20% less than wild-type mice and this lower weight persists until at least 7 months of age

behavior/neurological
• mutants are hypersensitive to PTZ-induced seizures, showing increased latency to clonic seizures, longer seizure duration, and shorter latency to lethality
• however, mice do not develop spontaneous seizures
• rearing activity of mutants did not habituate in the open field as in controls which show more rearings at the beginning than the end of the test session
• in the water maze, mice show a longer cumulative distance to target than controls during the learning period
• in a subsequent transfer test, mutants swim further from the target than wild-type mice
• reversal learning shows decreased ability to locate the platform
• in the second transfer test after reversal learning, mutants are closer to the previous platform location and further away from the new place than the wild-type mice
• however, mutants are able to learn the simple visually cued escape from the water maze
• in the elevated plus-maze, mice avoid entering the open arms almost completely, make less exploratory rearings and head dippings, and defecate more often than wild-type littermates
• although mice show normal exploration in the light-dark test, they show less rearings and defecate more frequently than wild-type mice
• mice are hyposensitive to tactile stimulation, with a lower percentage of mutants responding to a given stimulus force with calibrated von Frey hairs
• mice show less rearings during the elevated plus-maze, in the light-dark exploration test, and in the Y-maze test
• mice however, exhibit normal spontaneous locomotor activity and motor coordination in the open field, Y-maze, rotarod, and beam tests
• mice are hyposensitive to thermal stimulation, showing decreased sensitivity to lower temperatures in the tail withdrawal and hot-plate tests

nervous system
N
• mice do not show gross changes in brain histology
• mutants are hypersensitive to PTZ-induced seizures, showing increased latency to clonic seizures, longer seizure duration, and shorter latency to lethality
• however, mice do not develop spontaneous seizures

reproductive system
N
• mice are fertile




Genotype
MGI:5638537
ht3
Allelic
Composition
Slc12a5tm1Hsav/Slc12a5tm2Hsav
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc12a5tm1Hsav mutation (1 available); any Slc12a5 mutation (45 available)
Slc12a5tm2Hsav mutation (0 available); any Slc12a5 mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• dendritic protrusions are longer in neurons, however, no difference is protrusion density is seen





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory