Analysis Tools
cellular
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• MEFs treated with adeno-cre and later with tamoxifen
• increased cell proliferation
• more cells at the G2/M phase of the cell cycle
• foci formation in 3D cell culture after tamoxifen treatment
• withdrawal of tamoxifen reduces colony formation
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neoplasm
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• mice treated with adeno-cre at 6-8 weeks of age and tamoxifen starting three weeks later
• tamoxifen doses of 100ug/mouse, 250ug/mouse, or 1000ug/mouse resulted in no lung abnormalities
• the intermediate dose of 500ug/mouse results in lung hyperplasia starting after 5 weeks of treatment
• lung adenomas found after 8 weeks of treatment
• tamoxifen treatment discontinued after 12 weeks and lung hyperplasia is decreased a weeks later
• lungs are normal 2 weeks after discontinuation of tamoxifen treatment
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respiratory system
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• mice treated with adeno-cre at 6-8 weeks of age and tamoxifen starting three weeks later
• tamoxifen doses of 100ug/mouse, 250ug/mouse, or 1000ug/mouse resulted in no lung abnormalities
• the intermediate dose of 500ug/mouse results in lung hyperplasia starting after 5 weeks of treatment
• lung adenomas found after 8 weeks of treatment
• tamoxifen treatment discontinued after 12 weeks and lung hyperplasia is decreased a weeks later
• lungs are normal 2 weeks after discontinuation of tamoxifen treatment
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