Phenotypes associated with this allele

• Allele Symbol: Tgfbi^tm1Jwu
• Allele Name: targeted mutation 1, Jiangping Wu
• Allele ID: MGI:5638662
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tgfbi^tm1Jwu/Tgfbi^tm1Jwu	B6.129-Tgfbi^tm1Jwu	MGI:5792955
hm2	Tgfbi^tm1Jwu/Tgfbi^tm1Jwu	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5792953

Genotype
MGI:5792955

hm1

• Allelic Composition: Tgfbi^tm1Jwu/Tgfbi^tm1Jwu
• Genetic Background: B6.129-Tgfbi^tm1Jwu

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:213611 )

N • under normal conditions, mice are normoglycemic and show no differences in glucose tolerance, islet size or islet number relative to wild-type controls

increased pancreatic beta cell apoptosis ( J:213611 )

• after 24-h culture in serum-free medium, 53% of mutant pancreatic beta cells undergo apoptosis versus 38% of wild-type cells

abnormal pancreatic islet morphology ( J:213611 )

• after 24-h culture in serum-free medium, >25% of mutant islets lose their integrity (defined as 50% loss of the capsule surrounding the islets) and disintegrate relative to less than 5% of wild-type islets

decreased insulin secretion ( J:213611 )

• after 24-h culture in serum-free medium, mutant islets show a significantly lower ratio of high glucose- versus low glucose-stimulated insulin release than wild-type islets

homeostasis/metabolism

decreased insulin secretion ( J:213611 )

• after 24-h culture in serum-free medium, mutant islets show a significantly lower ratio of high glucose- versus low glucose-stimulated insulin release than wild-type islets

increased susceptibility to non-insulin-dependent diabetes ( J:213611 )

• mice are more susceptible to multiple low-dose streptozotocin (STZ)-induced diabetes than wild-type controls, as determined by blood glucose levels and % of mice that reach normoglycemia from day 6 to day 34 after STZ treatment

cellular

increased pancreatic beta cell apoptosis ( J:213611 )

• after 24-h culture in serum-free medium, 53% of mutant pancreatic beta cells undergo apoptosis versus 38% of wild-type cells

growth/size/body

growth/size/body region phenotype ( J:213611 )

N • contrary to expectation, mice are overtly normal with no obvious differences in body size relative to wild-type controls

Genotype
MGI:5792953

hm2

• Allelic Composition: Tgfbi^tm1Jwu/Tgfbi^tm1Jwu
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

endocrine/exocrine glands

abnormal pancreatic beta cell physiology ( J:213611 )

• mutant donor islets are inferior to wild-type donor islets in achieving normoglycemia when transplanted under the renal capsules of syngeneic mutant recipients which have been rendered diabetic by prior streptozotocin (STZ) treatment

• however, when mutant islets are transplanted into diabetic wild-type recipients they perform similarly to wild-type islets in terms of lowering blood glucose levels and rendering the recipients euglycemic