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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Nrastm1.1Nesh
targeted mutation 1.1, Norman E Sharpless
MGI:5645358
Summary 5 genotypes


Genotype
MGI:5752235
cn1
Allelic
Composition		 Cdkn2atm2.1Nesh/Cdkn2atm2.1Nesh
Nrastm1.1Nesh/Nrastm1.1Nesh
Tg(Tyr-cre/ERT2)13Bos/0
Genetic
Background		 B6J.Cg-Tg(Tyr-cre/ERT2)13Bos Nrastm1.1Nesh Cdkn2atm2.1Nesh
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdkn2atm2.1Nesh mutation (4 available); any Cdkn2a mutation (67 available)
Nrastm1.1Nesh mutation (2 available); any Nras mutation (44 available)
Tg(Tyr-cre/ERT2)13Bos mutation (12 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal melanocyte proliferation ( J:220627 )
• melanocytes treated with 4-hydroxytamoxifen (4-OHT) to induce cre activity exhibit decreased proliferation in culture and cease to proliferate after 2-3 passages

integument
increased cutaneous melanoma incidence ( J:220627 )
• mice treated with 4-OHT neonatally readily develop melanoma with 70% penetrance and a median latency of 26.3 weeks
• melanomas contain both spindle-cell and desmoplastic cell types with no overt signs of macrometastatic spread

pigmentation
abnormal melanocyte proliferation ( J:220627 )
• melanocytes treated with 4-hydroxytamoxifen (4-OHT) to induce cre activity exhibit decreased proliferation in culture and cease to proliferate after 2-3 passages
hyperpigmentation ( J:220627 )
• mice treated with 4-OHT neonatally exhibit the presence of nevi and hyperpigmented regions on the paws and tails

neoplasm
increased cutaneous melanoma incidence ( J:220627 )
• mice treated with 4-OHT neonatally readily develop melanoma with 70% penetrance and a median latency of 26.3 weeks
• melanomas contain both spindle-cell and desmoplastic cell types with no overt signs of macrometastatic spread

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
skin melanoma DOID:8923 OMIM:608035
OMIM:612263
 J:220627




Genotype
MGI:5752239
cn2
Allelic
Composition		 Cdkn2atm2.1Nesh/Cdkn2atm2.1Nesh
Nrastm1.1Nesh/Nrastm1.1Nesh
Stk11tm1.1Rdp/Stk11tm1.1Rdp
Tg(Tyr-cre/ERT2)13Bos/0
Genetic
Background		 B6J.Cg-Tg(Tyr-cre/ERT2)13Bos Nrastm1.1Nesh Cdkn2atm2.1Nesh Stk11tm1.1Rdp
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdkn2atm2.1Nesh mutation (4 available); any Cdkn2a mutation (67 available)
Nrastm1.1Nesh mutation (2 available); any Nras mutation (44 available)
Stk11tm1.1Rdp mutation (0 available); any Stk11 mutation (35 available)
Tg(Tyr-cre/ERT2)13Bos mutation (12 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
integument
increased cutaneous melanoma incidence ( J:220627 )
• mice treated with 4-hydroxytamoxifen (4-OHT) neonatally or in adulthood exhibit enhanced nevus formation
• mice treated with 4-OHT neonatally develop melanoma with 85% penetrance and a median latency of 22.1 weeks

neoplasm
increased cutaneous melanoma incidence ( J:220627 )
• mice treated with 4-hydroxytamoxifen (4-OHT) neonatally or in adulthood exhibit enhanced nevus formation
• mice treated with 4-OHT neonatally develop melanoma with 85% penetrance and a median latency of 22.1 weeks
increased metastatic potential ( J:220627 )
• 36% of mice treated with 4-OHT neonatally that develop melanoma show macrometastatic spread to lymph nodes, lung, spleen and/or liver

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
skin melanoma DOID:8923 OMIM:608035
OMIM:612263
 J:220627




Genotype
MGI:6393932
cn3
Allelic
Composition		 Ezh2tm1.1Nesh/Ezh2+
Nrastm1.1Nesh/Nras+
Tg(Tyr-cre/ERT2)13Bos/0
Genetic
Background		 involves: 129 * 129P2/OlaHsd * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ezh2tm1.1Nesh mutation (1 available); any Ezh2 mutation (72 available)
Nrastm1.1Nesh mutation (2 available); any Nras mutation (44 available)
Tg(Tyr-cre/ERT2)13Bos mutation (12 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
neoplasm ( J:239472 )
N
• tamoxifen-treated mice do not show acceleration of melanomagenesis and do not form melanoma compared to single Nras conditional mutants




Genotype
MGI:6393935
cn4
Allelic
Composition		 Cdkn2atm2.1Nesh/Cdkn2atm2.1Nesh
Ezh2tm1.1Nesh/Ezh2+
Nrastm1.1Nesh/Nras+
Tg(Tyr-cre/ERT2)13Bos/0
Genetic
Background		 involves: 129 * 129S4/SvJae * 129P2/OlaHsd * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdkn2atm2.1Nesh mutation (4 available); any Cdkn2a mutation (67 available)
Ezh2tm1.1Nesh mutation (1 available); any Ezh2 mutation (72 available)
Nrastm1.1Nesh mutation (2 available); any Nras mutation (44 available)
Tg(Tyr-cre/ERT2)13Bos mutation (12 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
increased melanoma incidence ( J:239472 )
• tamoxifen-treated mice develop melanoma similarly to conditional Nras and Cdkn2a double mutants




Genotype
MGI:6393936
cn5
Allelic
Composition		 Cdkn2atm2.1Nesh/Cdkn2atm2.1Nesh
Nrastm1.1Nesh/Nras+
Tg(Tyr-cre/ERT2)13Bos/0
Genetic
Background		 involves: 129 * 129S4/SvJae * 129P2/OlaHsd * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdkn2atm2.1Nesh mutation (4 available); any Cdkn2a mutation (67 available)
Nrastm1.1Nesh mutation (2 available); any Nras mutation (44 available)
Tg(Tyr-cre/ERT2)13Bos mutation (12 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
increased melanoma incidence ( J:239472 )
• tamoxifen-treated mice develop melanoma




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory