mortality/aging
• homozygous mutant embryos suffer a catastrophic event and die between E11.5 and E12.5
• lethality is associated with loss of cubilin protein expression and decreased expression of multiple genes implicated in the cubilin-megalin multi-ligand endocytic receptor complex at E10.5
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growth/size/body
• at E10.5, a small number of homozygous mutant embryos exhibit reduced size but are otherwise normal
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embryo
• at E10.5, a small number of homozygous mutant embryos exhibit reduced size but are otherwise normal
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cellular
• MEFs derived from E10.5 mutant embryos exhibit defects in transferrin internalization, uptake of low-density lipoprotein (LDL) receptor-related protein-1 (LRP1) cargo, and integrin alpha5-mediated fibronectin secretion
• mutant MEFs are resistant to LRP1-dependent cholix toxin cytotoxicity and internalize LDL poorly
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