Phenotypes associated with this allele

• Allele Symbol: Coprs^tm1.1Efab
• Allele Name: targeted mutation 1.1, Eric Fabbrizio
• Allele ID: MGI:5646194
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Coprs^tm1.1Efab/Coprs^tm1.1Efab	Not Specified	MGI:5792563

Genotype
MGI:5792563

hm1

• Allelic Composition: Coprs^tm1.1Efab/Coprs^tm1.1Efab
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

abnormal fat cell differentiation ( J:221154 )

• after induction of adipogenic differentiation with insulin and triiodothyronine, in vitro adipogenic conversion of mutant embryoid bodies (EBs) is markedly delayed at D4, and lipid droplets are rarely observed at D21, unlike in wild-type EB cultures

• after induction of adipogenic differentiation with insulin and rosiglitazone, in vitro adipogenic conversion of mutant primary embryonic fibroblasts (MEFs) is severely impaired, as determined by O Red Oil staining at D7

• surprisingly, transient overexpression of a human form of Copr5 fails to rescue the capacity of mutant MEFs to differentiate into adipocytes, as shown by O Red Oil staining at D6

decreased white fat cell number ( J:221154 )

• retroperitoneal white adipose tissue (WAT) contains fewer adipocytes than wild-type tissue

increased white fat cell size ( J:221154 )

• retroperitoneal WAT contains larger adipocytes than wild-type tissue

• altered adipocyte cellularity is due to Dlk-1 upregulation leading to a low pool of precursor cells that is able to differentiate into adipocytes

decreased retroperitoneal fat pad weight ( J:221154 )

• retroperitoneal fat weight is moderately but significantly reduced at 16 weeks of age relative to wild-type controls

• however, mean body weight is normal

abnormal white adipose tissue physiology ( J:221154 )

• the proliferation index of the vascular stromal fraction (a major source of progenitor cells in WAT) is reduced relative to wild-type controls, indicating a low pool of preadipocytes

• however, no difference is noted in mature adipocytes at 8 weeks of age

cellular

abnormal fat cell differentiation ( J:221154 )

• after induction of adipogenic differentiation with insulin and triiodothyronine, in vitro adipogenic conversion of mutant embryoid bodies (EBs) is markedly delayed at D4, and lipid droplets are rarely observed at D21, unlike in wild-type EB cultures

• after induction of adipogenic differentiation with insulin and rosiglitazone, in vitro adipogenic conversion of mutant primary embryonic fibroblasts (MEFs) is severely impaired, as determined by O Red Oil staining at D7

• surprisingly, transient overexpression of a human form of Copr5 fails to rescue the capacity of mutant MEFs to differentiate into adipocytes, as shown by O Red Oil staining at D6

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:221154 )

N • mice exhibit no differences in glucose and insulin tolerance tests relative to wild-type controls