Phenotypes associated with this allele

• Allele Symbol: Lancl1^tm1Pfw
• Allele Name: targeted mutation 1, Paul Worley
• Allele ID: MGI:5646272
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Lancl1^tm1Pfw/Lancl1^tm1.1Pfw Tg(Ddx4-cre)1Dcas/0	involves: 129 * C57BL/6 * FVB	MGI:7294208

Genotype
MGI:7294208

cn1

• Allelic Composition: Lancl1^tm1Pfw/Lancl1^tm1.1Pfw; Tg(Ddx4-cre)1Dcas/0
• Genetic Background: involves: 129 * C57BL/6 * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

decreased male germ cell number ( J:324139 )

♂ • at 8 weeks of age, the number of ACRV1+ germ cells (spermatids and spermatozoa) per tubule is significantly lower than that in control testes; the protein level of acrosin (an acrosome marker) is reduced

decreased testis weight ( J:324139 )

♂ • at 8 weeks of age, testis weight-to-body weight ratio is significantly lower than that in control males

♂ • however, body weight is normal

abnormal spermatogenesis ( J:324139 )

♂ • at 8 weeks of age, seminiferous tubules show obvious spermatogenic defects and a lower Johnsen's score, similar to Lancl1^tm1.1Pfw homozygous males

oligozoospermia ( J:324139 )

♂ • at 8 weeks of age, caudal epididymal sperm concentration is significantly lower than that in control males

reduced male fertility ( J:324139 )

♂

abnormal sperm physiology ( J:324139 )

♂ • spermatozoa exhibit significantly increased intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, indicating oxidative stress

decreased sperm progressive motility ( J:324139 )

♂ • at 8 weeks of age, sperm forward motility (%) is significantly lower than that in control males

asthenozoospermia ( J:324139 )

♂ • at 8 weeks of age, sperm motility (%) is significantly lower than that in control males

cellular

decreased male germ cell number ( J:324139 )

♂ • at 8 weeks of age, the number of ACRV1+ germ cells (spermatids and spermatozoa) per tubule is significantly lower than that in control testes; the protein level of acrosin (an acrosome marker) is reduced

oligozoospermia ( J:324139 )

♂ • at 8 weeks of age, caudal epididymal sperm concentration is significantly lower than that in control males

decreased sperm progressive motility ( J:324139 )

♂ • at 8 weeks of age, sperm forward motility (%) is significantly lower than that in control males

asthenozoospermia ( J:324139 )

♂ • at 8 weeks of age, sperm motility (%) is significantly lower than that in control males

oxidative stress ( J:324139 )

♂ • spermatozoa exhibit significantly increased intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, indicating oxidative stress

♂ • at 8 weeks of age, testes show an increased number of gammaH2AX+ cells in the seminiferous tubules and increased protein levels of 4-hydroxy-2-nonenal (4-HNE) and BAX, indicating oxidative damage in maturing male germ cells

endocrine/exocrine glands

decreased testis weight ( J:324139 )

♂ • at 8 weeks of age, testis weight-to-body weight ratio is significantly lower than that in control males

♂ • however, body weight is normal