Phenotypes associated with this allele

• Allele Symbol: F3^tm1Fct
• Allele Name: targeted mutation 1, Felix C Tanner
• Allele ID: MGI:5647876
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	F3^tm1Fct/F3^tm1Fct	involves: 129P2/OlaHsd * C57BL/6J	MGI:5696949

Genotype
MGI:5696949

hm1

• Allelic Composition: F3^tm1Fct/F3^tm1Fct
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Yolk sac abnormalities in F3^tm1Fct/F3^tm1Fct embryos

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:216519 )

• homozygotes die between E9.5 and E10.5

• all homozygotes are dead at E10.5

growth/size/body

embryonic growth retardation ( J:216519 )

• at E9.5, homozygotes display various stages of growth retardation and wasting

embryo

abnormal vitelline vasculature morphology ( J:216519 )

• although early yolk sac vessels develop normally up to E8.5, erythrocyte content, vessel wall thickness, and number of alpha-SMA expressing cells are reduced at E9.5

• by E10.5, mutant yolk sacs exhibit depletion of erythrocytes, a reduced number of alpha-SMA positive cells, thin-walled capillaries, and partial detachment of mesodermal from endodermal layer, unlike wild-type controls

• no intact blood filled vessels are detected in mutant yolk sac en face preparations at E10.5

embryonic growth retardation ( J:216519 )

• at E9.5, homozygotes display various stages of growth retardation and wasting

abnormal extraembryonic mesoderm development ( J:216519 )

• at E10.5, mutant yolk sacs exhibit a low number of alpha-smooth-muscle-actin (alpha-SMA) positive cells and partial separation of mesodermal from endodermal layer

homeostasis/metabolism

abnormal blood coagulation ( J:216519 )

• a plasma clotting assay revealed that no measurable procoagulant activity is detected in mutant E9.5 whole embryos

hematopoietic system

abnormal embryonic erythrocyte morphology ( J:216519 )

• at E9.5, erythrocyte content is reduced in mutant yolk sacs

• by E10.5, mutant yolk sac vessels lack erythrocytes

cardiovascular system

abnormal vitelline vasculature morphology ( J:216519 )

• although early yolk sac vessels develop normally up to E8.5, erythrocyte content, vessel wall thickness, and number of alpha-SMA expressing cells are reduced at E9.5

• by E10.5, mutant yolk sacs exhibit depletion of erythrocytes, a reduced number of alpha-SMA positive cells, thin-walled capillaries, and partial detachment of mesodermal from endodermal layer, unlike wild-type controls

• no intact blood filled vessels are detected in mutant yolk sac en face preparations at E10.5