Phenotypes associated with this allele

• Allele Symbol: Tg(tetO-Hgf,-EGFP)24Tcre
• Allele Name: transgene insertion 24, Tiziana Crepaldi
• Allele ID: MGI:5648118
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs Pax7^tm1Pgr/Pax7^tm1Pgr Tg(CKMM-tTA)A3Rhvh/0 Tg(tetO-Hgf,-EGFP)24Tcre/0	involves: 129S2/SvPas * 129X1/SvJ * FVB	MGI:5882413
cx2	Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs Pax7^tm1Pgr/Pax7^+ Tg(CKMM-tTA)A3Rhvh/0 Tg(tetO-Hgf,-EGFP)24Tcre/0	involves: 129S2/SvPas * 129X1/SvJ * FVB	MGI:5882414
cx3	Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs Tg(CKMM-tTA)A3Rhvh/0 Tg(tetO-Hgf,-EGFP)24Tcre/0	involves: 129X1/SvJ * FVB	MGI:5882410
cx4	Cdkn2a^tm1Cjs/Cdkn2a^+ Tg(CKMM-tTA)A3Rhvh/0 Tg(tetO-Hgf,-EGFP)24Tcre/0	involves: 129X1/SvJ * FVB	MGI:5882411
cx5	Tg(CKMM-tTA)A3Rhvh/0 Tg(tetO-Hgf,-EGFP)24Tcre/0	involves: FVB	MGI:5882409

Genotype
MGI:5882413

cx1

• Allelic Composition: Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs; Pax7^tm1Pgr/Pax7^tm1Pgr; Tg(CKMM-tTA)A3Rhvh/0; Tg(tetO-Hgf,-EGFP)24Tcre/0
• Genetic Background: involves: 129S2/SvPas * 129X1/SvJ * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

muscle

increased rhabdomyosarcoma incidence ( J:237183 )

• 29% of tumors are embryonal rhabdomyosarcoma

neoplasm

increased sarcoma incidence ( J:237183 )

• 87% of mice develop sarcomas

• 71% of tumors that form in mice are undifferentiated pleomorphic sarcoma

increased rhabdomyosarcoma incidence ( J:237183 )

• 29% of tumors are embryonal rhabdomyosarcoma

Genotype
MGI:5882414

cx2

• Allelic Composition: Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs; Pax7^tm1Pgr/Pax7⁺; Tg(CKMM-tTA)A3Rhvh/0; Tg(tetO-Hgf,-EGFP)24Tcre/0
• Genetic Background: involves: 129S2/SvPas * 129X1/SvJ * FVB

muscle

increased rhabdomyosarcoma incidence ( J:237183 )

• mice develop mainly embryonal rhabdomyosarcoma, with 92% of tumors being embryonal rhabdomyosarcoma

neoplasm

increased sarcoma incidence ( J:237183 )

• 100% of mice develop sarcoma

• 8% of tumors are undifferentiated pleomorphic sarcoma

increased rhabdomyosarcoma incidence ( J:237183 )

• mice develop mainly embryonal rhabdomyosarcoma, with 92% of tumors being embryonal rhabdomyosarcoma

Genotype
MGI:5882410

cx3

• Allelic Composition: Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs; Tg(CKMM-tTA)A3Rhvh/0; Tg(tetO-Hgf,-EGFP)24Tcre/0
• Genetic Background: involves: 129X1/SvJ * FVB

muscle

skeletal muscle hyperplasia ( J:237183 )

• 32% of mice that develop embryonal rhabdomyosarcoma exhibit muscle hyperplasia while 68% of mice develop embryonal rhabdomyosarcoma without muscle hyperplasia

increased rhabdomyosarcoma incidence ( J:237183 )

• all mice develop tumors, mainly multi-step embryonal rhabdomyosarcoma with a short latency of 3.95 months

• embryonal rhabdomyosarcoma originates from satellite cells

• treatment of mice with doxycycline when tumors become palpable does not impair tumor growth

• mice maintained under doxycycline treatment until P10 develop tumors after doxycycline removal

neoplasm

increased rhabdomyosarcoma incidence ( J:237183 )

• all mice develop tumors, mainly multi-step embryonal rhabdomyosarcoma with a short latency of 3.95 months

• embryonal rhabdomyosarcoma originates from satellite cells

• treatment of mice with doxycycline when tumors become palpable does not impair tumor growth

• mice maintained under doxycycline treatment until P10 develop tumors after doxycycline removal

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
embryonal rhabdomyosarcoma		DOID:3246	OMIM:268210	J:237183

Genotype
MGI:5882411

cx4

• Allelic Composition: Cdkn2a^tm1Cjs/Cdkn2a⁺; Tg(CKMM-tTA)A3Rhvh/0; Tg(tetO-Hgf,-EGFP)24Tcre/0
• Genetic Background: involves: 129X1/SvJ * FVB

muscle

increased rhabdomyosarcoma incidence ( J:237183 )

• mice develop multi-step embryonal rhabdomyosarcoma with a latency of 6 months

• majority of tumors lose the wild-type allele

neoplasm

increased rhabdomyosarcoma incidence ( J:237183 )

• mice develop multi-step embryonal rhabdomyosarcoma with a latency of 6 months

• majority of tumors lose the wild-type allele

Genotype
MGI:5882409

cx5

• Allelic Composition: Tg(CKMM-tTA)A3Rhvh/0; Tg(tetO-Hgf,-EGFP)24Tcre/0
• Genetic Background: involves: FVB

muscle

abnormal skeletal muscle fiber morphology ( J:237183 )

• myofibers contain more myonuclei than controls

decreased skeletal muscle fiber size ( J:237183 )

• mice exhibit a modest reduction of muscle fiber size

• however, mice show normal postnatal muscle growth

increased satellite cell number ( J:237183 )

• mice exhibit a higher number of satellite cells compared to controls

• MyoD-positive cells are seen in muscle sections indicating presence of activated satellite cells