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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Mirc32tm1.1Jtm
targeted mutation 1.1, Joshua T Mendell
MGI:5648483
Summary 2 genotypes


Genotype
MGI:5707652
cn1
Allelic
Composition		 Mirc32tm1.1Jtm/Mirc32tm1.1Jtm
Twist2tm1(cre)Dor/Twist2+
Genetic
Background		 B6.129(Cg)-Twist2tm1.1(cre)Dor Mirc32tm1.1Jtm
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mirc32tm1.1Jtm mutation (1 available); any Mirc32 mutation (3 available)
Twist2tm1(cre)Dor mutation (0 available); any Twist2 mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal intestinal mucosa morphology ( J:214382 )
• lamina propia myofibroblasts within the ulcers of DSS treated (+ 2 days recovery) mice are disorganized and fail to elongate along the basal-luminal axis
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice
intestinal ulcer ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
abnormal colon morphology ( J:214382 )
• colons from DSS treated (+ 2 days recovery) mice remain hemorrhagic , necrotic and lack hyper proliferative crypts
colonic necrosis ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
impaired intestine regeneration ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice
• pH3+ (a mitotic marker) cells are restricted to crypt base and are reduced in number
increased susceptibility to induced colitis ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice

endocrine/exocrine glands
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice

immune system
increased susceptibility to induced colitis ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice

mortality/aging
abnormal susceptibility to induced morbidity/mortality ( J:214382 )
• 50% of mice die within 3 days following the completion of 5 days (D5+3) of DSS treatment
• control mice exhibit almost complete recovery of intestinal mucosal architecture 9 days after DSS treatment




Genotype
MGI:5707654
cn2
Allelic
Composition		 Mirc32tm1.1Jtm/Mirc32tm1.1Jtm
Tg(Myh11-cre,-EGFP)2Mik/?
Genetic
Background		 B6.Cg-Mirc32tm1.1Jtm Tg(Myh11-cre,-EGFP)2Mik
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mirc32tm1.1Jtm mutation (1 available); any Mirc32 mutation (3 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal intestinal mucosa morphology ( J:214382 )
• lamina propia myofibroblasts within the ulcers of DSS treated (+ 2 days recovery) mice are abnormal
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration
intestinal ulcer ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
abnormal colon morphology ( J:214382 )
• colons from DSS treated (+ 2 days recovery) mice remain hemorrhagic and necrotic
• however, phenotype is less severe than in Mirc32tm1.2Jtm mice
colonic necrosis ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
impaired intestine regeneration ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration
• pH3+ cells are reduced in number
increased susceptibility to induced colitis ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration

endocrine/exocrine glands
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration

immune system
increased susceptibility to induced colitis ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
12/17/2024
MGI 6.24 		The Jackson Laboratory