About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Mirc32tm1.1Jtm
targeted mutation 1.1, Joshua T Mendell
MGI:5648483
Summary 2 genotypes


Genotype
MGI:5707652
cn1
Allelic
Composition		 Mirc32tm1.1Jtm/Mirc32tm1.1Jtm
Twist2tm1(cre)Dor/Twist2+
Genetic
Background		 B6.129(Cg)-Twist2tm1.1(cre)Dor Mirc32tm1.1Jtm
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mirc32tm1.1Jtm mutation (1 available); any Mirc32 mutation (3 available)
Twist2tm1(cre)Dor mutation (0 available); any Twist2 mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal intestinal mucosa morphology ( J:214382 )
• lamina propia myofibroblasts within the ulcers of DSS treated (+ 2 days recovery) mice are disorganized and fail to elongate along the basal-luminal axis
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice
intestinal ulcer ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
abnormal colon morphology ( J:214382 )
• colons from DSS treated (+ 2 days recovery) mice remain hemorrhagic , necrotic and lack hyper proliferative crypts
colonic necrosis ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
impaired intestine regeneration ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice
• pH3+ (a mitotic marker) cells are restricted to crypt base and are reduced in number
increased susceptibility to induced colitis ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice

endocrine/exocrine glands
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice

immune system
increased susceptibility to induced colitis ( J:214382 )
• intestinal crypts do not regenerate in DSS treated (+ 2 days recovery) mice

mortality/aging
abnormal susceptibility to induced morbidity/mortality ( J:214382 )
• 50% of mice die within 3 days following the completion of 5 days (D5+3) of DSS treatment
• control mice exhibit almost complete recovery of intestinal mucosal architecture 9 days after DSS treatment




Genotype
MGI:5707654
cn2
Allelic
Composition		 Mirc32tm1.1Jtm/Mirc32tm1.1Jtm
Tg(Myh11-cre,-EGFP)2Mik/?
Genetic
Background		 B6.Cg-Mirc32tm1.1Jtm Tg(Myh11-cre,-EGFP)2Mik
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mirc32tm1.1Jtm mutation (1 available); any Mirc32 mutation (3 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal intestinal mucosa morphology ( J:214382 )
• lamina propia myofibroblasts within the ulcers of DSS treated (+ 2 days recovery) mice are abnormal
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration
intestinal ulcer ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
abnormal colon morphology ( J:214382 )
• colons from DSS treated (+ 2 days recovery) mice remain hemorrhagic and necrotic
• however, phenotype is less severe than in Mirc32tm1.2Jtm mice
colonic necrosis ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
impaired intestine regeneration ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration
• pH3+ cells are reduced in number
increased susceptibility to induced colitis ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration

endocrine/exocrine glands
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration

immune system
increased susceptibility to induced colitis ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
08/02/2024
MGI 6.24 		The Jackson Laboratory