Phenotypes associated with this allele

• Allele Symbol: Ephb1^tm1Dgen
• Allele Name: targeted mutation 1, Deltagen
• Allele ID: MGI:5659739
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ephb1^tm1Dgen/Ephb1^tm1Dgen	Not Specified	MGI:5767281
cx2	Ephb1^tm1Dgen/Ephb1^+ Fezf2^tm1Skm/Fezf2^tm1Skm	involves: 129	MGI:5767283
cx3	Ephb1^tm1Dgen/Ephb1^tm1Dgen Fezf2^tm1Skm/Fezf2^+	involves: 129	MGI:5767284

Genotype
MGI:5767281

hm1

• Allelic Composition: Ephb1^tm1Dgen/Ephb1^tm1Dgen
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal axon fasciculation ( J:214959 )

• internal capsule defasciculation at P28

abnormal axon guidance ( J:214959 )

• at P28, cortical neurons aberrantly project through the anterior commissure, unlike in wild-type brains

• ectopic axon bundles extend within the internal capsule toward the external capsule and continue to grow ventrally

• high-angular-resolution diffusion imaging (HARDI) tractography confirmed that axon fibers in dorsal areas of the neocortex merge with the anterior commissure, unlike in wild-type brains

• however, class-specific identity and distribution of cortical projection neurons is unaltered

increased anterior commissure size ( J:214959 )

• expansion of the anterior commissure at P28

increased brain external capsule size ( J:214959 )

• expansion of the brain external capsule at P28

abnormal brain internal capsule morphology ( J:214959 )

• at P28, the internal capsule contains smaller than wild type and partly unfasciculated axon bundles

decreased brain internal capsule size ( J:214959 )

• internal capsule reduction at P28

abnormal somatosensory cortex morphology ( J:214959 )

• at P2, DiI anterograde injections in deep layers of the somatosensory cortex revealed axons ectopically crossing at the anterior commissure and extending ventrally and rostrally, unlike in wild-type mice

cellular

abnormal axon fasciculation ( J:214959 )

• internal capsule defasciculation at P28

abnormal axon guidance ( J:214959 )

• at P28, cortical neurons aberrantly project through the anterior commissure, unlike in wild-type brains

• ectopic axon bundles extend within the internal capsule toward the external capsule and continue to grow ventrally

• high-angular-resolution diffusion imaging (HARDI) tractography confirmed that axon fibers in dorsal areas of the neocortex merge with the anterior commissure, unlike in wild-type brains

• however, class-specific identity and distribution of cortical projection neurons is unaltered

Genotype
MGI:5767283

cx2

• Allelic Composition: Ephb1^tm1Dgen/Ephb1⁺; Fezf2^tm1Skm/Fezf2^tm1Skm
• Genetic Background: involves: 129

nervous system

abnormal axon guidance ( J:214959 )

• at P7, PLAP-positive axons inappropriately project through the anterior commissure, unlike in wild-type brains

• no PLAP-positive axons are found in the cerebral peduncle or spinal cord

cellular

abnormal axon guidance ( J:214959 )

• at P7, PLAP-positive axons inappropriately project through the anterior commissure, unlike in wild-type brains

• no PLAP-positive axons are found in the cerebral peduncle or spinal cord

Genotype
MGI:5767284

cx3

• Allelic Composition: Ephb1^tm1Dgen/Ephb1^tm1Dgen; Fezf2^tm1Skm/Fezf2⁺
• Genetic Background: involves: 129

nervous system

abnormal axon guidance ( J:214959 )

• at P7, a large proportion of PLAP-positive axons extended aberrantly to contralateral targets, crossing the midline via an enlarged anterior commissure, unlike in control mice where PLAP-positive axons are exclusively observed extending along ipsilateral, corticofugal trajectories

• a reduction of PLAP-positive axons is found in the cerebral peduncle and the cervical spinal cord, canonical targets of the corticospinal tract in wild-type mice

increased anterior commissure size ( J:214959 )

• enlarged anterior commissure at P7

decreased corticospinal tract size ( J:214959 )

• at P7

cellular

abnormal axon guidance ( J:214959 )

• at P7, a large proportion of PLAP-positive axons extended aberrantly to contralateral targets, crossing the midline via an enlarged anterior commissure, unlike in control mice where PLAP-positive axons are exclusively observed extending along ipsilateral, corticofugal trajectories

• a reduction of PLAP-positive axons is found in the cerebral peduncle and the cervical spinal cord, canonical targets of the corticospinal tract in wild-type mice