Phenotypes associated with this allele

• Allele Symbol: Tbck^{tm1b(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:5661955
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tbck^tm1b(EUCOMM)Hmgu/Tbck^tm1b(EUCOMM)Hmgu	B6J.B6N-Tbck^tm1b(EUCOMM)Hmgu	MGI:7581833
hm2	Tbck^tm1b(EUCOMM)Hmgu/Tbck^tm1b(EUCOMM)Hmgu	C57BL/6N-Tbck^tm1b(EUCOMM)Hmgu/J	MGI:6263648
ht3	Tbck^tm1b(EUCOMM)Hmgu/Tbck^+	B6J.B6N-Tbck^tm1b(EUCOMM)Hmgu	MGI:7581835

Genotype
MGI:7581833

hm1

• Allelic Composition: Tbck^{tm1b(EUCOMM)Hmgu}/Tbck^{tm1b(EUCOMM)Hmgu}
• Genetic Background: B6J.B6N-Tbck^{tm1b(EUCOMM)Hmgu}
• Cell Lines: HEPD0678_1_D08

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality ( J:344893 )

• homozygotes backcrossed to C57BL/6J for six generations are embryonic lethal

Genotype
MGI:6263648

hm2

• Allelic Composition: Tbck^{tm1b(EUCOMM)Hmgu}/Tbck^{tm1b(EUCOMM)Hmgu}
• Genetic Background: C57BL/6N-Tbck^{tm1b(EUCOMM)Hmgu}/J
• Cell Lines: HEPD0678_1_D08

Data Sources

IMPC Data for Tbck

mortality/aging

preweaning lethality, incomplete penetrance ( J:211773 )

IMPC - JAX

Genotype
MGI:7581835

ht3

• Allelic Composition: Tbck^{tm1b(EUCOMM)Hmgu}/Tbck⁺
• Genetic Background: B6J.B6N-Tbck^{tm1b(EUCOMM)Hmgu}
• Cell Lines: HEPD0678_1_D08

behavior/neurological

behavior/neurological phenotype ( J:344893 )

N • mice exhibit normal balance and motor coordination on an accelerating rotatrod assay and show no significant differences in the overall acoustic startle response (ASR), although females respond less to stimuli of increasing decibels than wild-type controls

decreased exploration in new environment ( J:344893 )

• in a Y-maze assay, males show a significantly lower number of total arm entries than wild-type males at the 8 min timepoint; however, the number of spontaneous arm alternations is normal at all timepoints in both sexes

• in an elevated zero maze (EZM) test, females travel significantly less than males and strongly trend towards less total distance traveled than wild-type females

• however, time spent in the open areas of the EZM is not significantly altered, indicating normal anxiety-like behavior

decreased vertical activity ( J:344893 )

• in the open field test, mice rear significantly less than wild-type controls over a 10-min period, with males more severely affected than females

• however, open field total activity, horizontal activity, and center activity are normal in both sexes

nervous system

nervous system phenotype ( J:344893 )

N • regardless of genotype, male mice show significant reductions in phospho-S6 (235/236) and p-4E-BP1 relative to female controls, indicating decreased mTORC1 signaling in male brains

decreased prepulse inhibition ( J:344893 )

• mice show a significant reduction of PPI at the 87-dB pre-pulse stimulus

• however, no differences are detected at the 78 or 81-dB pre-pulse stimuli