Phenotypes associated with this allele

• Allele Symbol: Nmt1^tm1.1Poru
• Allele Name: targeted mutation 1.1, Stefan Porubsky
• Allele ID: MGI:5696555
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nmt1^tm1.1Poru/Nmt1^tm1.1Poru Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2 * SJL	MGI:5696656
cn2	Nmt1^tm1.1Poru/Nmt1^tm1.1Poru Nmt2^tm1.1Poru/Nmt2^tm1.1Poru Tg(Lck-cre)1Cwi/0	involves: C57BL/6 * DBA/2 * SJL	MGI:5696659

Genotype
MGI:5696656

cn1

• Allelic Composition: Nmt1^tm1.1Poru/Nmt1^tm1.1Poru; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell number ( J:226234 )

• moderate in the thymus

• in the blood, lymph nodes and spleen

decreased double-positive T cell number ( J:226234 )

• at 2 and 6 months, but less so than in conditional Nmt1^tm1.1Poru and Nmt2^tm1.1Poru knock-out mice

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells

abnormal CD4-positive, alpha beta T cell morphology ( J:226234 )

• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

abnormal CD8-positive, alpha beta T cell morphology ( J:226234 )

• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

hematopoietic system

decreased T cell number ( J:226234 )

• moderate in the thymus

• in the blood, lymph nodes and spleen

decreased double-positive T cell number ( J:226234 )

• at 2 and 6 months, but less so than in conditional Nmt1^tm1.1Poru and Nmt2^tm1.1Poru knock-out mice

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells

abnormal CD4-positive, alpha beta T cell morphology ( J:226234 )

• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

abnormal CD8-positive, alpha beta T cell morphology ( J:226234 )

• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

Genotype
MGI:5696659

cn2

• Allelic Composition: Nmt1^tm1.1Poru/Nmt1^tm1.1Poru; Nmt2^tm1.1Poru/Nmt2^tm1.1Poru; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

immune system phenotype ( J:226234 )

N • mice do not exhibit any signs of autoimmune disease

increased T cell apoptosis ( J:226234 )

• during all stages of T cell development except DN1 that does not express the cre transgene

• 10-fold in double positive cells

decreased T cell proliferation ( J:226234 )

• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice

decreased splenocyte proliferation ( J:226234 )

• when stimulated with IL2

small thymus medulla ( J:226234 )

• however, cortical area is normal

increased double-negative T cell number ( J:226234 )

increased gamma-delta T cell number ( J:226234 )

• almost 3-fold

decreased T cell number ( J:226234 )

• in the thymus more so than single conditional knock-outs

• in the blood, lymph nodes and spleen as in Nmt1^tm1.1Poru conditional knock-outs

decreased DN4 thymocyte number ( J:226234 )

• at 6 months

decreased double-positive T cell number ( J:226234 )

• at 2 and 6 months more so than in either single conditional knock-out

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells at 2 and 6 months

• following treatment with CpC-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

abnormal CD4-positive, alpha beta T cell morphology ( J:226234 )

• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

abnormal CD8-positive, alpha beta T cell morphology ( J:226234 )

• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

decreased circulating interferon-gamma level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased circulating interleukin-2 level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased circulating tumor necrosis factor level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased interferon-gamma secretion ( J:226234 )

• after TCR-independent activation

decreased interleukin-2 secretion ( J:226234 )

• after TCR-independent activation

decreased susceptibility to endotoxin shock ( J:226234 )

• mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B exhibit reduced plasma levels of TNF, IL2 and IFN-gamma compared with wild-type mice

homeostasis/metabolism

decreased circulating interferon-gamma level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased circulating interleukin-2 level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

decreased circulating tumor necrosis factor level ( J:226234 )

• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

cellular

increased T cell apoptosis ( J:226234 )

• during all stages of T cell development except DN1 that does not express the cre transgene

• 10-fold in double positive cells

decreased T cell proliferation ( J:226234 )

• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice

decreased splenocyte proliferation ( J:226234 )

• when stimulated with IL2

endocrine/exocrine glands

small thymus medulla ( J:226234 )

• however, cortical area is normal

decreased DN4 thymocyte number ( J:226234 )

• at 6 months

hematopoietic system

increased T cell apoptosis ( J:226234 )

• during all stages of T cell development except DN1 that does not express the cre transgene

• 10-fold in double positive cells

decreased T cell proliferation ( J:226234 )

• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice

decreased splenocyte proliferation ( J:226234 )

• when stimulated with IL2

small thymus medulla ( J:226234 )

• however, cortical area is normal

increased double-negative T cell number ( J:226234 )

increased gamma-delta T cell number ( J:226234 )

• almost 3-fold

decreased T cell number ( J:226234 )

• in the thymus more so than single conditional knock-outs

• in the blood, lymph nodes and spleen as in Nmt1^tm1.1Poru conditional knock-outs

decreased DN4 thymocyte number ( J:226234 )

• at 6 months

decreased double-positive T cell number ( J:226234 )

• at 2 and 6 months more so than in either single conditional knock-out

decreased single-positive T cell number ( J:226234 )

• CD4+ and CD8+ single positive cells at 2 and 6 months

• following treatment with CpC-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice

abnormal CD4-positive, alpha beta T cell morphology ( J:226234 )

• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes

abnormal CD8-positive, alpha beta T cell morphology ( J:226234 )

• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes