Phenotypes associated with this allele

• Allele Symbol: Evc2^tm2.1Mis
• Allele Name: targeted mutation 2.1, Yuji Mishina
• Allele ID: MGI:5697358
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Evc2^tm2.1Mis/Evc2^tm2.1Mis Tg(Mpz-cre)94Imeg/0	involves: 129X1/SvJ	MGI:5698050
cn2	Evc2^tm2.1Mis/Evc2^tm2.1Mis Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:5698047
cn3	Evc2^tm2.1Mis/Evc2^tm2.1Mis Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129X1/SvJ * CD-1	MGI:5698049
cn4	Evc2^tm2.1Mis/Evc2^tm2.1Mis Tg(Col2a1-cre/ERT)KA3Smac/0	involves: 129X1/SvJ * FVB/N	MGI:5698048

Genotype
MGI:5698050

cn1

• Allelic Composition: Evc2^tm2.1Mis/Evc2^tm2.1Mis; Tg(Mpz-cre)94Imeg/0
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

craniofacial

short lower incisors ( J:226455 )

• lower incisors showed slightly shorter length and relatively normal radio density

short upper incisors ( J:226455 )

• upper incisors were shorter than those in control littermates

abnormal tooth development ( J:226455 )

• at E18.5, pre-ameloblasts located in the posterior area of normal upper incisor are less differentiated with fully extended nuclei within ameloblasts

• at E18.5 deposition of extracellular matrix proteins surrounding ameloblasts is reduced

abnormal ameloblast morphology ( J:226455 )

• at E18.5 revealed that ameloblasts in mutant mice did not polarize appropriately when compared to those with control littermates

skeleton

short lower incisors ( J:226455 )

• lower incisors showed slightly shorter length and relatively normal radio density

short upper incisors ( J:226455 )

• upper incisors were shorter than those in control littermates

abnormal tooth development ( J:226455 )

• at E18.5, pre-ameloblasts located in the posterior area of normal upper incisor are less differentiated with fully extended nuclei within ameloblasts

• at E18.5 deposition of extracellular matrix proteins surrounding ameloblasts is reduced

abnormal ameloblast morphology ( J:226455 )

• at E18.5 revealed that ameloblasts in mutant mice did not polarize appropriately when compared to those with control littermates

growth/size/body

short lower incisors ( J:226455 )

• lower incisors showed slightly shorter length and relatively normal radio density

short upper incisors ( J:226455 )

• upper incisors were shorter than those in control littermates

abnormal tooth development ( J:226455 )

• at E18.5, pre-ameloblasts located in the posterior area of normal upper incisor are less differentiated with fully extended nuclei within ameloblasts

• at E18.5 deposition of extracellular matrix proteins surrounding ameloblasts is reduced

abnormal ameloblast morphology ( J:226455 )

• at E18.5 revealed that ameloblasts in mutant mice did not polarize appropriately when compared to those with control littermates

mortality/aging

mortality/aging ( J:226455 )

N • did not notice lethality in the neural crest-specific mutant mice during the period of observation (up to 6 months)

Genotype
MGI:5698047

cn2

• Allelic Composition: Evc2^tm2.1Mis/Evc2^tm2.1Mis; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA

growth/size/body

disproportionate dwarf ( J:226455 )

• disproportionally shorter forelimbs and hindlimbs are seen

Genotype
MGI:5698049

cn3

• Allelic Composition: Evc2^tm2.1Mis/Evc2^tm2.1Mis; Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
• Genetic Background: involves: 129X1/SvJ * CD-1

growth/size/body

growth/size/body region phenotype ( J:226455 )

N • no overt differences in body weight compared to controls

skeleton

skeleton phenotype ( J:226455 )

N • no overt phenotypes in skeletal system at 6 months after birth compared to controls

N • no overt differences in lengths of long bones compared to controls and no overt changes in trabecular components and cortical components of tibia

Genotype
MGI:5698048

cn4

• Allelic Composition: Evc2^tm2.1Mis/Evc2^tm2.1Mis; Tg(Col2a1-cre/ERT)KA3Smac/0
• Genetic Background: involves: 129X1/SvJ * FVB/N

growth/size/body

disproportionate dwarf ( J:226455 )

• skeletal staining with alizarin red and alcian blue indicated a decrease of limb length

limbs/digits/tail

short humerus ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short radius ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short ulna ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short femur ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short fibula ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short tibia ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

skeleton

short humerus ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short radius ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short ulna ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short femur ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short fibula ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls

short tibia ( J:226455 )

• length of appendicular bone indicated 710% decrease for each bone when compared to that of controls