Phenotypes associated with this allele

• Allele Symbol: Brinp1^tm1Imts
• Allele Name: targeted mutation 1, Ichiro Matsuoka
• Allele ID: MGI:5698819
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Brinp1^tm1Imts/Brinp1^tm1Imts	B6J.Cg-Brinp1^tm1Imts	MGI:5699609

Genotype
MGI:5699609

hm1

• Allelic Composition: Brinp1^tm1Imts/Brinp1^tm1Imts
• Genetic Background: B6J.Cg-Brinp1^tm1Imts

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:221295 )

• adult homozygotes have a body weight that is ~85% of that in wild-type controls

• however, adult brain and hippocampus weights remain normal

behavior/neurological

behavior/neurological phenotype ( J:221295 )

N • homozygotes show no significant differences in their sensory abilities (hot plate test), neuromuscular strength (forelimb grip strength in Newton), vertical activity and stereotypic behavior relative to wild-type controls

abnormal spatial working memory ( J:221295 )

• in a T-maze alteration test, homozygotes show a significantly lower % of correct responses than wild-type controls at the certain blocks of 3 sessions during repetitive sessions with a short delay period (3 s)

• when task difficulty is raised by prolonging the delay period (>30 s), homozygotes show lower performances, suggesting a mild deficit in working memory

decreased anxiety-related response ( J:221295 )

• in the open field test, homozygotes spend significantly more time than wild-type controls in the center area of the apparatus during the first 60 min of the test

• in the light/dark transition test, homozygotes travel 1.54- and 1.34-fold longer distances than wild-type controls in the light and dark boxes, respectively, show a 1.62-fold increase in the number of transitions between dark and light boxes, and tend to spend more time in the light box than wild-type controls

• in the elevated plus maze test, homozygotes show a 1.43-fold longer travel distance, enter 1.82-fold more frequently into the open arms, and spend 2.21-fold longer time in the open arms than wild-type controls

impaired coordination ( J:221295 )

• homozygotes show a markedly short latency in the wire hang test and a significantly shorter latency to fall from the rotating drum in a Rotarod test

• however, homozygotes improve their performance during repeating trials of the Rotarod test in a manner similar to that of wild-type controls

increased locomotor activity ( J:221295 )

• in the home cage, homozygotes show significantly higher locomotor activity during night time than wild-type controls

• in the open field test, homozygotes show >1.5 times higher locomotor/exploratory activity than wild-type controls throughout the 120 min observation period

abnormal social investigation ( J:221295 )

• in the social interaction test in a new environment, the total duration of contacts and the mean duration per contact are 61% and 50% less than those of wild-type controls, respectively, suggesting poor social interaction

• however, homozygotes showed a normal physical contact pattern in the home cage

nervous system

impaired neuron differentiation ( J:221295 )

• analysis of neuronal differentiation of BrdU-incorporated cells suggests that excess newborn neurons in the subgranular zone (SGZ) of the dentate gyrus continue to differentiate to some extent (i.e. to doublecortin-positive immature neurons) until 2 weeks after their birth, but fail to differentiate further or survive thereafter

abnormal brain interneuron morphology ( J:221295 )

• homozygotes show a 1.65-fold increase in the number of parvalbumin expressing interneurons in the hippocampal CA1 subregion relative to wild-type controls

abnormal hippocampus CA1 region morphology ( J:221295 )

• homozygotes show a 1.65-fold increase in the number of parvalbumin expressing interneurons in the hippocampal CA1 subregion relative to wild-type controls

abnormal dentate gyrus subgranular zone morphology ( J:221295 )

• homozygotes show increased adult neurogenesis in the subgranular zone (SGZ) of the dentate gyrus resulting in a more immature neuronal population in the granule cell layer

• at 7 and 8 weeks of age, the average number of BrdU-positive cells per section in the SGZ is 1.65- and 1.94-fold greater than in wild-type controls, respectively, with a similar increase noted for Ki-67-positive cells

increased neuronal precursor cell number ( J:221295 )

• at 7 weeks of age, staining of doublecortin (a marker for neuroblast and immature neurons) is stronger in the mutant dentate gyrus than that in wild-type controls

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:221295 )

N • homozygotes exhibit normal body temperature

cellular

impaired neuron differentiation ( J:221295 )

• analysis of neuronal differentiation of BrdU-incorporated cells suggests that excess newborn neurons in the subgranular zone (SGZ) of the dentate gyrus continue to differentiate to some extent (i.e. to doublecortin-positive immature neurons) until 2 weeks after their birth, but fail to differentiate further or survive thereafter

skeleton

skeleton phenotype ( J:221295 )

N • homozygotes exhibit a normal skeleton