cellular
• the numbers of chromocenters per nucleus in the ventricular zone are greatly decreased; this is due to hyperclustering of chromocenters and not due to loss of chromocenters
• hyperclustering of chromocenters occurs in neural stem cells and in postmitotic neurons
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• structure of nucleoli within the interphase nucleus is largely disorganized and the number of nucleoli per nucleus in the ventricular zone is decreased
• decrease in number of nucleoli occurs in neural stem cells and in postmitotic neurons
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• neural stem cells show unusually large, Hoechst-dense structures at prophase, however the kinetics of mitotic progression is normal
• chromosome segregation defects at anaphase and postmitotic cells at E16.5
• defects in mitotic chromosome architecture and segregation are more prominent than in mice conditionally deleted for Ncaph
• the numbers of chromocenters (the nuclear structure that forms from the association of pericentric heterochromatin from several different chromosomes during interphase) per nucleus in the ventricular zone are greatly decreased; this is due to hyperclustering of chromocenters and not due to loss of chromocenters
• hyperclustering of chromocenters occurs in neural stem cells and in postmitotic neurons
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nervous system
• apoptotic cells are seen in the cortical plate
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• cerebral cortices are highly disorganized by E19.5
• some apoptosis is seen in the developing cortices at E13.5
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• numbers of neural stem cells are decreased at E16.5
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• numbers of neurons are decreased at E16.5
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• neural stem cells undergo apoptosis in the ventricular zone at E16.5
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