Phenotypes associated with this allele

• Allele Symbol: Appl2^tm1.1Test
• Allele Name: targeted mutation 1.1, Joseph Testa
• Allele ID: MGI:5703918
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Appl2^tm1.1Test/Appl2^tm1.1Test	Not Specified	MGI:5766634
cx2	Appl1^tm1.2Test/Appl1^tm1.2Test Appl2^tm1.1Test/Appl2^tm1.1Test	involves: 129X1/SvJ	MGI:5766636

Genotype
MGI:5766634

hm1

• Allelic Composition: Appl2^tm1.1Test/Appl2^tm1.1Test
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:227340 )

N • at 8 weeks of age, mice exhibit normal numbers and % of lymphocytes, monocytes, neutrophils, eosinophils, and basophils relative to controls

N • no pernicious anemia is observed, as WBC counts and spleen and liver size are normal

increased hematocrit ( J:227340 )

• hematocrit is significantly increased, esp. in female mice

decreased mean corpuscular hemoglobin concentration ( J:227340 )

• MCHC is significantly decreased, esp. in female mice

increased mean corpuscular volume ( J:227340 )

• MCV is significantly increased, esp. in female mice

cellular

abnormal cell physiology ( J:227340 )

• primary MEFs derived from E13.5 embryos exhibit decreased Akt activation upon stimulation with HGF; in contrast, FBS- or EGF-induced Akt activation is relatively normal

• in vivo, basal Akt activity is not significantly altered in stomach, liver or muscle tissues

decreased fibroblast cell migration ( J:227340 )

• primary MEFs derived from E13.5 embryos exhibit reduced HGF-induced transmembrane migration and decreased movement in a wound-healing assay as well as markedly decreased HGF-triggered invasion in a matrigel assay

• in contrast, EGF-induced migration and invasion are normal

Genotype
MGI:5766636

cx2

• Allelic Composition: Appl1^tm1.2Test/Appl1^tm1.2Test; Appl2^tm1.1Test/Appl2^tm1.1Test
• Genetic Background: involves: 129X1/SvJ

mortality/aging

mortality/aging ( J:227340 )

N • unexpectedly, mice are viable and show grossly normal postnatal growth and fertility, similar to single homozygotes

hematopoietic system

hematopoietic system phenotype ( J:227340 )

N • at 8 weeks of age, mice exhibit normal numbers and % of lymphocytes, monocytes, neutrophils, eosinophils, and basophils relative to controls

N • no pernicious anemia is observed, as WBC counts and spleen and liver size are normal

N • T cell development and Pi3k-Akt signaling in thymic T cells are normal

increased hematocrit ( J:227340 )

• hematocrit is significantly increased, esp. in female mice

decreased mean corpuscular hemoglobin concentration ( J:227340 )

• MCHC is significantly decreased, esp. in female mice

increased mean corpuscular volume ( J:227340 )

• MCV is significantly increased, esp. in female mice

cellular

abnormal cell physiology ( J:227340 )

• primary MEFs derived from E13.5 embryos exhibit decreased Akt activation upon stimulation with HGF; in contrast, FBS- or EGF-induced Akt activation is relatively normal

• in vivo, basal Akt activity is not significantly altered in stomach, liver or muscle tissues

decreased fibroblast cell migration ( J:227340 )

• primary MEFs derived from E13.5 embryos exhibit reduced HGF-induced transmembrane migration and decreased movement in a wound-healing assay as well as markedly decreased HGF-triggered invasion in a matrigel assay

• in contrast, EGF-induced migration and invasion are normal