Analysis Tools
digestive/alimentary system
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• at 3 days, 1 week, 4 weeks and 8 weeks after 4-hydroxytamoxifen (4-OHT) injection, adult mice exhibit no differences in crypt or villus morphology in the small intestine relative to control littermates
• in culture, 4-OHT-treated small intestinal crypt organoids show reduced Wntless (Wls) protein levels but exhibit normal overall morphology, with Paneth cells visible at the tips of the bud, and can grow for many passages without Wnt supplementation in the growth medium
• 4-OHT-treated intestinal organoids show normal survival rates in different R-spondin concentrations
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• 5 days after passaging, 4-OHT-treated intestinal organoids develop fewer crypt-like buds relative to control organoids, indicating a mild proliferation defect
• supplementation of exogenous Wnt3a in the medium does not fully rescue this growth defect, although 4-OHT-treated organoids can respond to Wnt signaling
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cellular
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• 5 days after passaging, 4-OHT-treated intestinal organoids develop fewer crypt-like buds relative to control organoids, indicating a mild proliferation defect
• supplementation of exogenous Wnt3a in the medium does not fully rescue this growth defect, although 4-OHT-treated organoids can respond to Wnt signaling
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