Phenotypes associated with this allele

• Allele Symbol: Irs4^tm1.1Mfw
• Allele Name: targeted mutation 1.1, Morris F White
• Allele ID: MGI:5705105
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Irs2^tm2Mfw/Irs2^tm2Mfw Irs4^tm1.1Mfw/Y Lepr^tm3(cre)Mgmj/Lepr^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5705239
cn2	Irs2^tm2Mfw/Irs2^tm2Mfw Irs4^tm1.1Mfw/Y Tg(Nes-cre)1Kln/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:5705238
ot3	Irs4^tm1.1Mfw/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5705236

Genotype
MGI:5705239

cn1

• Allelic Composition: Irs2^tm2Mfw/Irs2^tm2Mfw; Irs4^tm1.1Mfw/Y; Lepr^tm3(cre)Mgmj/Lepr⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:221293 )

♂N • blood glucose levels are similar to controls

growth/size/body

growth/size/body region phenotype ( J:221293 )

♂N • body weight is essentially similar to homozygous Irs2^tm2Mfw / heterozygous Lepr^tm3(cre)Mgmj male mice

Genotype
MGI:5705238

cn2

• Allelic Composition: Irs2^tm2Mfw/Irs2^tm2Mfw; Irs4^tm1.1Mfw/Y; Tg(Nes-cre)1Kln/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

growth/size/body

increased body weight ( J:221293 )

♂ • 60% heavier than controls

♂ • 20% heavier than Irs2^tm2Mfw / Tg(Nes-cre)1Kln male mice

adipose tissue

abnormal adipose tissue amount ( J:221293 )

♂ • increased adipose tissue relative to Irs2^tm2Mfw / Tg(Nes-cre)1Kln male mice

increased brown fat cell lipid droplet size ( J:221293 )

♂ • larger lipid filled vacuoles in brown adipose tissue compared to Irs2^tm2Mfw / Tg(Nes-cre)1Kln male mice

♂ • resembles white adipose tissue

liver/biliary system

hepatic steatosis ( J:221293 )

♂

homeostasis/metabolism

increased circulating leptin level ( J:221293 )

♂ • greatly elevated

decreased oxygen consumption ( J:221293 )

♂

abnormal glucose homeostasis ( J:221293 )

♂

increased circulating glucose level ( J:221293 )

♂ • ad libitum feeding results in increased blood glucose

increased fasting circulating glucose level ( J:221293 )

♂ • dramatically elevated at 3 months

increased circulating insulin level ( J:221293 )

♂ • 5 fold elevation

impaired glucose tolerance ( J:221293 )

♂ • dramatically impaired at 3 months

insulin resistance ( J:221293 )

♂ • at 3 months and persisting to at least 9 months

decreased basal metabolism ( J:221293 )

♂ • reduced heat production

behavior/neurological

increased food intake ( J:221293 )

♂ • food intake increased 10% more than in Irs2^tm2Mfw / Tg(Nes-cre)1Kln male mice

Genotype
MGI:5705236

ot3

• Allelic Composition: Irs4^tm1.1Mfw/Y
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:221293 )

♂N • normal leptin levels

♂N • normal fasted glucose levels at 3 months

♂N • normal glucose tolerance

♂N • normal insulin levels

increased circulating glucose level ( J:221293 )

♂ • ad libitum feeding results in insignificantly increased blood glucose

growth/size/body

growth/size/body region phenotype ( J:221293 )

♂N • normal size

mortality/aging

mortality/aging ( J:221293 )

♂N • no embryonic lethality

reproductive system

reproductive system phenotype ( J:221293 )

N • fertile (both sexes)