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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Mrps18ctm1.1(KOMP)Vlcg
targeted mutation 1.1, Velocigene
MGI:5705873
Summary 3 genotypes


Genotype
MGI:6262930
hm1
Allelic
Composition
Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg
Genetic
Background
B6N(Cg)-Mrps18ctm1.1(KOMP)Vlcg/J
Cell Lines 12639A-D8
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mrps18ctm1.1(KOMP)Vlcg mutation (1 available); any Mrps18c mutation (10 available)
Data Sources
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging




Genotype
MGI:6376484
hm2
Allelic
Composition
Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg
Genetic
Background
C57BL/6N-Mrps18ctm1.1(KOMP)Vlcg/JMmucd
Cell Lines 12639A-D8
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mrps18ctm1.1(KOMP)Vlcg mutation (1 available); any Mrps18c mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg mice exhibit embryonic lethality, with embryos recovered at E7.5 but not at E9.5. Embryos are smaller and form a rudimentary egg cylinder but no primitive streak or hallmarks of gastrulation are seen at E7.5.

mortality/aging
• although homozygous embryos are recovered at expected Mendelian ratios at E7.5, decidua with embryo resorptions are detected at E8.5 (J:290315)
• homozygous embryos are recovered in expected Mendelian ratios at E7.5 but arrest prior to gastrulation; no homozygous embryos are recovered at E9.5 (J:279207)

embryo
• no structures typical of gastrulation are observed at E7.5 (J:290315)
• no morphological hallmarks typical of gastrulating embryos are observed at E7.5 (J:279207)
• embryos stall at about the size of wild-type E6.0 embryos, just prior to the onset of gastrulation; only two cell layers with bilaterally symmetric egg-cylinder morphology are seen at E7.5 (J:290315)
• however, no disorganization or pyknotic/dying cells are apparent at E7.5 (J:290315)
• embryos arrest prior to gastrulation (J:279207)
• at E7.5, embryos are significantly delayed and appear as relatively pristine early egg-cylinder stage embryos
• however, no widespread active Trp53-positive apoptotic cells are detected at E7.5
• at E7.5, embryos are dramatically smaller than controls (J:290315)
• embryos are much smaller than controls at E7.5 (J:279207)
• at E7.5, embryos still retain robust nuclear Pou5f/Oct4 signal in the epiblast
• a rudimentary egg cylinder is observed at E7.5
• no morphological head folds are evident at E7.5 (J:290315)
• no head folds are present at E7.5 (J:279207)
• no morphological nodes are evident at E7.5 (J:290315)
• no embryonic node is present at E7.5 (J:279207)
• no morphological primitive streak or Brachyury (T)-positive cells are detected at E7.5 (J:290315)
• no morphological evidence of primitive streak formation is observed at E7.5 (J:279207)
• no Brachyury (T)-positive cells are present at E7.5, suggesting failure of primitive streak initiation (J:279207)
• no morphological allantois is evident at E7.5

growth/size/body
• at E7.5, embryos are significantly delayed and appear as relatively pristine early egg-cylinder stage embryos
• however, no widespread active Trp53-positive apoptotic cells are detected at E7.5
• at E7.5, embryos are dramatically smaller than controls (J:290315)
• embryos are much smaller than controls at E7.5 (J:279207)

cellular
N
• embryos show no significant differences in 4HNE (4-hydroxynonenal) levels or localization relative to wild-type controls, suggesting no increase in lipid peroxidation or oxidative stress
• embryos show a significantly higher ADP to ATP ratio, indicating mitochondrial dysfunction
• at E7.5, TEM shows a marked alteration in mitochondrial morphology, with many large vesicle-like structures present within every mitochondrion
• however, the ratio of mitochondrial to nuclear genome content is similar to that in control embryos and only a slight increase in caspase-3 mediated apoptosis is noted in the embryonic portion
• mitochondria exhibit far fewer cristae at E7.5
• embryo sections show increased numbers of bright pH3 foci (pH3, a marker of dividing cells) relative to controls, suggesting that cells arrest in the G2/late G2 phase
• embryos show a significantly higher ADP to ATP ratio, indicating mitochondrial dysfunction
• mitochondrial translation is impaired leading to a lack of ETC related proteins, as indicated by the absence of MT-CO2 protein (mitochondrially encoded cytochrome c oxidase II) in embryonic cells
• absence of functional ETC components is likely responsible for the drastic reduction of ATP production

homeostasis/metabolism
• embryos show a significantly higher ADP to ATP ratio, indicating mitochondrial dysfunction




Genotype
MGI:6262929
ht3
Allelic
Composition
Mrps18ctm1.1(KOMP)Vlcg/Mrps18c+
Genetic
Background
B6N(Cg)-Mrps18ctm1.1(KOMP)Vlcg/J
Cell Lines 12639A-D8
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mrps18ctm1.1(KOMP)Vlcg mutation (1 available); any Mrps18c mutation (10 available)
Data Sources
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system

homeostasis/metabolism





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory