Analysis Tools
homeostasis/metabolism
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• in vitro, insulin release following stimulation with low (2.8 mM) and high glucose (16.7 mM) during a 30-min incubation is significantly reduced in isolated pancreatic islets relative wild-type controls
• both the initial insulin release peak and the sustained insulin release are significantly decreased following stimulation with a high glucose concentration (16.7mM) relative wild-type controls
• transcriptome analysis of mutant pancreatic islets revealed significantly altered expression of components involved in insulin secretion
• however, gross pancreatic islet morphology, beta-cell mass, and intracellular insulin content in lysed islets are normal
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• on a standard chow diet, 16-weeks-old mice tend to have lower serum insulin levels after intraperitoneal glucose application relative wild-type controls
• after 12 weeks of western type diet, mice show significantly lower glucose-stimulated insulin secretion at both 40 and 60 min after intraperitoneal glucose injection relative wild-type controls
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• under a western type diet, glucose tolerance is significantly impaired following oral and intraperitoneal glucose application relative to wild-type controls
• however, insulin sensitivity appears unaffected
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endocrine/exocrine glands
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• in vitro, insulin release following stimulation with low (2.8 mM) and high glucose (16.7 mM) during a 30-min incubation is significantly reduced in isolated pancreatic islets relative wild-type controls
• both the initial insulin release peak and the sustained insulin release are significantly decreased following stimulation with a high glucose concentration (16.7mM) relative wild-type controls
• transcriptome analysis of mutant pancreatic islets revealed significantly altered expression of components involved in insulin secretion
• however, gross pancreatic islet morphology, beta-cell mass, and intracellular insulin content in lysed islets are normal
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growth/size/body
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• mice fed a western type diet for 3 months gain significantly less weight than similarly fed wild-type controls
• however, no differences in body weight development are observed under a standard chow diet
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• at 3 months of age, spleen weight is significantly increased
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digestive/alimentary system
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• following oral application of dye-labeled dextran, excretion of blue dextran in feces is significantly delayed relative to wild-type controls
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cardiovascular system
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• at 3 months of age, heart rate is increased (P < 0.01)
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• at 3 months of age, mean arterial pressure is significantly increased (P < 0.001)
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• at 3 months of age, diastolic pressure is significantly increased (P < 0.001)
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• at 3 months of age, systolic pressure is increased (P < 0.01)
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respiratory system
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• reduced airway responsiveness as shown by invasive plethysmography tests
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• significantly increased dynamic compliance in response to increasing inhaled doses (10-80 mg/ml) of methacholine
• however, inhaled UDP-glucose (100 uM) has no significant effect on lung resistance and dynamic compliance
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hematopoietic system
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• at 3 months of age, spleen weight is significantly increased
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immune system
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• at 3 months of age, spleen weight is significantly increased
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