Phenotypes associated with this allele

• Allele Symbol: Tle4^tm1.2Dasw
• Allele Name: targeted mutation 1.2, David A Sweetser
• Allele ID: MGI:5707932
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tle4^tm1.2Dasw/Tle4^tm1.2Dasw	B6.129S6(Cg)-Tle4^tm1.2Dasw	MGI:5707943

Genotype
MGI:5707943

hm1

• Allelic Composition: Tle4^tm1.2Dasw/Tle4^tm1.2Dasw
• Genetic Background: B6.129S6(Cg)-Tle4^tm1.2Dasw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:219243 )

• mice die by 4 weeks of life

immune system

immune system phenotype ( J:219243 )

N • CD11b+ cell numbers are normal in peripheral blood

increased thymocyte apoptosis ( J:219243 )

• in the thymus at 3 weeks

abnormal thymus morphology ( J:219243 )

• loss of demarcation between the cortex and medulla

thymus atrophy ( J:219243 )

• by 3 weeks of age

thymus hypoplasia ( J:219243 )

• by 3 weeks of age

abnormal lymphopoiesis ( J:219243 )

• reduced common lymphoid progenitors

decreased pre-pro B cell number ( J:219243 )

• reduced pre/pro-B fractions A through C

increased NK T cell number ( J:219243 )

• CD3+ cells in the bone marrow

• however, numbers are normal in peripheral blood

decreased leukocyte cell number ( J:219243 )

• severe by 4 weeks of age

decreased lymphocyte cell number ( J:219243 )

• severe by 4 weeks of age in the peripheral blood

• 5-fold in the bone marrow

decreased DN2 thymocyte number ( J:219243 )

• by 3 weeks of age

decreased double-positive T cell number ( J:219243 )

• by 3 weeks of age

decreased B cell number ( J:219243 )

• in peripheral blood and bone marrow

decreased follicular B cell number ( J:219243 )

• by 3 weeks of age

spleen hypoplasia ( J:219243 )

• by 3 weeks of age with reduced B cells and CD11b+ cells

decreased spleen B cell follicle number ( J:219243 )

• almost complete loss by 3 weeks of age

skeleton

abnormal long bone epiphyseal plate morphology ( J:219243 )

• at P21 to P28

abnormal long bone epiphyseal plate proliferative zone ( J:219243 )

• with shorter columns of cells

abnormal long bone hypertrophic chondrocyte zone ( J:219243 )

• with shorter columns of cells

decreased long bone epiphyseal plate size ( J:219243 )

• thinner with shorter columns of cells in both the proliferating and hypertrophic zones by 3 weeks of age

decreased bone mineral content ( J:219243 )

• in trabecular and cortical bone

• in both membranous and endochondral bone

decreased compact bone thickness ( J:219243 )

decreased trabecular bone mass ( J:219243 )

• almost absent in the tibia and femur at P21 to P28

growth/size/body

decreased body weight ( J:219243 )

postnatal growth retardation ( J:219243 )

• growth plateaus at the 2 week of life

cellular

increased thymocyte apoptosis ( J:219243 )

• in the thymus at 3 weeks

endocrine/exocrine glands

increased thymocyte apoptosis ( J:219243 )

• in the thymus at 3 weeks

abnormal thymus morphology ( J:219243 )

• loss of demarcation between the cortex and medulla

thymus atrophy ( J:219243 )

• by 3 weeks of age

thymus hypoplasia ( J:219243 )

• by 3 weeks of age

decreased DN2 thymocyte number ( J:219243 )

• by 3 weeks of age

hematopoietic system

increased thymocyte apoptosis ( J:219243 )

• in the thymus at 3 weeks

abnormal thymus morphology ( J:219243 )

• loss of demarcation between the cortex and medulla

thymus atrophy ( J:219243 )

• by 3 weeks of age

thymus hypoplasia ( J:219243 )

• by 3 weeks of age

decreased common myeloid progenitor cell number ( J:219243 )

abnormal lymphopoiesis ( J:219243 )

• reduced common lymphoid progenitors

decreased pre-pro B cell number ( J:219243 )

• reduced pre/pro-B fractions A through C

decreased bone marrow cell number ( J:219243 )

• 2-fold at 2 weeks of age

increased NK T cell number ( J:219243 )

• CD3+ cells in the bone marrow

• however, numbers are normal in peripheral blood

decreased leukocyte cell number ( J:219243 )

• severe by 4 weeks of age

decreased lymphocyte cell number ( J:219243 )

• severe by 4 weeks of age in the peripheral blood

• 5-fold in the bone marrow

decreased DN2 thymocyte number ( J:219243 )

• by 3 weeks of age

decreased double-positive T cell number ( J:219243 )

• by 3 weeks of age

decreased B cell number ( J:219243 )

• in peripheral blood and bone marrow

decreased follicular B cell number ( J:219243 )

• by 3 weeks of age

decreased hematopoietic stem cell number ( J:219243 )

• almost 4-fold with a 3-fold reduction in CD34+ LKS cells

• however, the number of long-term hematopoietic cells is normal

spleen hypoplasia ( J:219243 )

• by 3 weeks of age with reduced B cells and CD11b+ cells

decreased spleen B cell follicle number ( J:219243 )

• almost complete loss by 3 weeks of age

abnormal hematopoietic stem cell physiology ( J:219243 )

• increased apoptotic cells

• impaired ability to form long term colonies

• intrinsic impairment in B cell differentiation and exhaustion after serial transplantation

• however, cell proliferation is normal