Phenotypes associated with this allele

• Allele Symbol: Adgrb3^tm1Ksak
• Allele Name: targeted mutation 1, Kenji Sakimura
• Allele ID: MGI:5708584
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Adgrb3^tm1Ksak/Adgrb3^tm1Ksak Tg(Pcp2-cre)2Mpin/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:5896611

Genotype
MGI:5896611

cn1

• Allelic Composition: Adgrb3^tm1Ksak/Adgrb3^tm1Ksak; Tg(Pcp2-cre)2Mpin/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:219706 )

N • normal cerebellar foliation and lamination of cortical structures

abnormal neuron morphology ( J:219706 )

• reduced number of vGluT2+ puncta in the cerebella

• climbing fiber terminals penetrate to a shallower depth than in wild-type mice

• however, transforming Purkinje cells with a wild-type form of the gene re-establishes climbing fiber synapse elimination

abnormal afterhyperpolarization ( J:219706 )

• reduced complex spikes from Purkinje cells

• reduced climbing fiber-evoked afterhyperpolarization and suppression of spontaneous spikes

abnormal single cell response ( J:219706 )

• whole-cell patch-clamp recordings show reduced one-to-one relationship of Purkinje cells with climbing fibers and smaller amplitude of strongest climbing fiber excitatory postsynaptic currents

• however, transforming Purkinje cells with a wild-type form (but not one lacking a domain required for interaction with C1ql1) of the gene re-establishes increases amplitude of strongest climbing fiber excitatory postsynaptic currents

absent long-term depression ( J:219706 )

• simultaneously activating parallel fibers and climbing fibers for 2 min at 1 Hz in the current-clamp mode fails to develop long term depression

behavior/neurological

behavior/neurological phenotype ( J:219706 )

N • mice exhibit no ataxia and normal motor coordination on the rotor-rod test

abnormal motor learning ( J:219706 )

• severely impaired adaptation of the horizontal optokinetic response

• however, horizontal optokinetic response prior to training is normal