Phenotypes associated with this allele

• Allele Symbol: Abcb10^tm1.2Tafu
• Allele Name: targeted mutation 1.2, Tatsuhiko Furukawa
• Allele ID: MGI:5708657
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Abcb10^tm1.2Tafu/Abcb10^tm1.2Tafu	involves: C57BL/6NCrlj * CBA/JNCrlj	MGI:5708664
cn2	Abcb10^tm1.1Tafu/Abcb10^tm1.2Tafu Tg(Mx1-cre)1Cgn/0	involves: C57BL/6J * C57BL/6NCrlj * CBA/J * CBA/JNCrlJ	MGI:5749139

Genotype
MGI:5708664

hm1

• Allelic Composition: Abcb10^tm1.2Tafu/Abcb10^tm1.2Tafu
• Genetic Background: involves: C57BL/6NCrlj * CBA/JNCrlj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

oxidative stress ( J:223428 )

• reactive oxygen species accumulation in embryos

embryo

abnormal embryonic hematopoiesis ( J:223428 )

• embryonic hematopoietic failure

hematopoietic system

abnormal blood cell morphology ( J:223428 )

• hemocytes do not contain heme and contain free iron in the mitochondria

• cell membranes and the cytoplasms of hemocytes are fragmented at E11.5

abnormal embryonic hematopoiesis ( J:223428 )

• embryonic hematopoietic failure

abnormal hemoglobin ( J:223428 )

• decrease in heme level in E11.5 embryos

• benzidine-positive cells are not present in E11.5 embryos indicating absence of hemoglobinized cells in the heart and liver

homeostasis/metabolism

abnormal iron level ( J:223428 )

• embryos exhibit iron accumulation in circulating cells

• hemocytes show accumulation of iron in the mitochondria

integument

pallor ( J:223428 )

• embryos at E11.5 are paler due to the absence of hemoglobinized cells in the heart and fetal liver

liver/biliary system

abnormal liver morphology ( J:223428 )

• E11.5 liver shows wide spaces between cells, indicating reduced cell-cell interactions

abnormal hepatocyte morphology ( J:223428 )

• cell membranes and the cytoplasms of hepatocytes are fragmented at E11.5

abnormal liver physiology ( J:223428 )

• fetal livers show an increase in apoptosis at E11.5

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:223428 )

• embryonic lethality between E10.5 and E11.5

Genotype
MGI:5749139

cn2

• Allelic Composition: Abcb10^tm1.1Tafu/Abcb10^tm1.2Tafu; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6J * C57BL/6NCrlj * CBA/J * CBA/JNCrlJ

cellular

abnormal mitochondrial morphology ( J:223428 )

• bone marrow cell mitochondria of pI-pC treated mice show prominent contours of the membranes and are swollen

• accumulation of iron in mitochondria of bone marrow cells of pI-pC treated mice

hematopoietic system

abnormal erythropoiesis ( J:223428 )

• pI-pC treated mice show impaired erythroid cell maturation

hypochromic microcytic anemia ( J:223428 )

• pI-pC treated mice develop anemia; anemia is classified as microcytic and hypochromic type

decreased erythrocyte cell number ( J:223428 )

• pI-pC treated mice exhibit a decrease in red blood cell number

abnormal hemoglobin ( J:223428 )

• heme is reduced in the peripheral blood of pI-pC treated mice

decreased hemoglobin content ( J:223428 )

• pI-pC treated mice show a decrease in hemoglobin

• however, white blood cell number and platelet count are normal

decreased mean corpuscular hemoglobin concentration ( J:223428 )

• low mean corpuscular hemoglobin concentration in pI-pC treated mice

decreased mean corpuscular volume ( J:223428 )

• low mean corpuscular volume in pI-pC treated mice

reticulocytosis ( J:223428 )

• pI-pC treated mice show an increase in the number of reticulocytes

homeostasis/metabolism

abnormal iron level ( J:223428 )

• pI-pC treated mice exhibit accumulation of iron in mitochondria