Phenotypes associated with this allele

• Allele Symbol: Prdx3^tm1Hjha
• Allele Name: targeted mutation 1, Hunjoo Ha
• Allele ID: MGI:5749424
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prdx3^tm1Hjha/Prdx3^tm1Hjha	involves: 129S4/SvJae * C57BL/6	MGI:5781002

Genotype
MGI:5781002

hm1

• Allelic Composition: Prdx3^tm1Hjha/Prdx3^tm1Hjha
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

increased body fat mass ( J:228860 )

• increased fat mass by 20 months of age

increased body weight ( J:228860 )

• body weight is normal at 4 months but significantly increased by 20 months of age

adipose tissue

increased white adipose tissue amount ( J:228860 )

• increased white adipose tissue amount (WAT) at 4 and 20 months of age

increased body fat mass ( J:228860 )

• increased fat mass by 20 months of age

abnormal white fat cell differentation ( J:228860 )

• increased adipogenic gene expression in differentiated adipocyte-derived stem cells (ASCs) isolated from subcutaneous fat pads of mutant mice relative to wild-type controls

increased white fat cell size ( J:228860 )

• white fat cell hypertrophy at 4 and 20 months of age

abnormal epididymal fat pad morphology ( J:228860 )

• significant increase in epididymal WAT by 20 months of age

abnormal renal fat pad morphology ( J:228860 )

• significant increase in perirenal fat by 20 months of age

abnormal white adipose tissue physiology ( J:228860 )

• downregulation of adiponectin mRNA and protein expression in mutant adipocytes isolated from epididymal WAT

• upregulation of plasminogen activator inhibitor-1 (PAI-1) mRNA and protein expression in mutant adipocytes isolated from epididymal WAT

cellular

abnormal white fat cell differentation ( J:228860 )

• increased adipogenic gene expression in differentiated adipocyte-derived stem cells (ASCs) isolated from subcutaneous fat pads of mutant mice relative to wild-type controls

abnormal mitochondrial physiology ( J:228860 )

• severe downregulation of mitochondrial proteins involved in oxidative phosphorylation and fatty acid metabolism in mutant adipocytes isolated from epididymal WAT relative to wild-type controls

• impaired mitochondrial biogenesis in mutant adipocytes as shown by a significant reduction in mtDNA and a trend towards downregulation of genes related to mitochondrial biosynthesis

• increased mitochondrial protein carbonylation in mutant subcutaneous, epididymal, and omental WAT relative to wild-type controls

decreased fatty acid oxidation ( J:228860 )

• downregulation of mitochondrial enzymes involved in fatty acid oxidation in mutant adipocytes, suggesting impaired ability to burn excessive fat

abnormal redox activity ( J:228860 )

• aberrant mitochondrial redox state resulting in abnormal adipocyte differentiation

oxidative stress ( J:228860 )

• increased mitochondrial oxidative stress as shown by downregulation of antioxidant genes, including manganese superoxide dismutase, heme oxygenase-1 and quinone oxidoreductase 1, in mutant adipocytes relative to wild-type controls

• increased oxidative stress as shown by increased plasma lipid peroxidation levels (measured as thiobarbituric acid reactive substance levels) and HSP60/nitrotyrosine staining in WAT relative to wild-type controls

• increased mitochondrial protein carbonylation in mutant subcutaneous, epididymal, and omental WAT relative to wild-type controls

homeostasis/metabolism

decreased fatty acid oxidation ( J:228860 )

• downregulation of mitochondrial enzymes involved in fatty acid oxidation in mutant adipocytes, suggesting impaired ability to burn excessive fat

increased fasting circulating glucose level ( J:228860 )

• increased plasma glucose levels in the fasted state, with no change in fed glucose levels, relative to wild-type controls

increased circulating insulin level ( J:228860 )

• increased plasma insulin levels in the fasted state relative to wild-type controls

increased circulating cholesterol level ( J:228860 )

• significantly increased total plasma cholesterol levels relative to wild-type controls

decreased circulating triglyceride level ( J:228860 )

• trend towards lower plasma triglyceride levels, likely due to increased level of Lpl mRNA levels in mutant adipocytes

abnormal circulating protein level ( J:228860 )

• significantly increased plasma plasminogen activator inhibitor-1 (PAI-1) levels at 20 months of age

impaired glucose tolerance ( J:228860 )

• mice are glucose intolerant in an oral glucose tolerance test

insulin resistance ( J:228860 )

• mice show significantly decreased sensitivity to insulin in an insulin tolerance test

decreased adiponectin level ( J:228860 )

• downregulation of adiponectin mRNA and protein expression in mutant adipocytes isolated from epididymal WAT

• in contrast, adipocyte leptin levels remain normal

decreased circulating adiponectin level ( J:228860 )

• significantly decreased plasma adiponectin levels at 20 months of age

• in contrast, plasma leptin levels remain normal

abnormal lipid metabolism ( J:228860 )

• increased lipid metabolism in mutant adipocytes as shown by upregulation of genes related to lipid homeostasis