embryo
• epiblast cells form a smaller than normal outgrowth colony after 3 days in culture
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• inner cell mass (ICM) cells form a smaller than normal outgrowth colony after 3 days in culture
• however, blastocysts hatch from the zona pellucida with apparent trophectoderm and primitive endoderm cells
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mortality/aging
IMPC - JAX
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IMPC - JAX
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• normal looking blastocysts are present at E3.5 (36% versus expected 25%, n=11); no embryos are recovered at E7.5 with an excess number of resorption sites
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