Analysis Tools
mortality/aging
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• homozygous mutant embryos died between E10.0 and E10.5
• no newborn homozygotes were obtained from heterozygote intercrosses
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growth/size/body
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• at E9.5
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embryo
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• absence of nodal cilia at E8.0
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• absence of nodal flow due to loss of nodal cilia
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• homozygotes displayed defective left-right axis patterning, as shown by ectopic bilateral expression of Lefty-1 at E8.5
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• at E9.5
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• at E9.5
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• the cranial neural tube failed to close at E9.5
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• at E10.5
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cardiovascular system
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• at E9.5
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• 2 of 9 homozygotes showed heart looping defects at E9.5
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• 1 of 9 homozygotes showed left-right reversed heart looping
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• 1 of 9 homozygotes showed a linear heart tube at E9.5
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• pericardial edema at E10.5
• a variable degree of pericardial edema was noted in all embryos with inverted heart looping
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hemorrhage
(
J:221138
)
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• at E9.5
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nervous system
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• at E9.5
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• the cranial neural tube failed to close at E9.5
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• at E10.5
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homeostasis/metabolism
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• pericardial edema at E10.5
• a variable degree of pericardial edema was noted in all embryos with inverted heart looping
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• severe cardiac edema at E10.5
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cellular
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• absence of nodal cilia at E8.0
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• absence of nodal flow due to loss of nodal cilia
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