Phenotypes associated with this allele

• Allele Symbol: Nlgn1^tm1.1Sud
• Allele Name: targeted mutation 1.1, Thomas C Sudhof
• Allele ID: MGI:5754559
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud Nlgn3^tm4.1Sud/Y Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac	MGI:5754948
cn2	Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac	MGI:5754949
cn3	Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud Nlgn3^tm4.1Sud/Y Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac	MGI:5754963

Genotype
MGI:5754948

cn1

• Allelic Composition: Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud; Nlgn3^tm4.1Sud/Y; Tg(Pcp2-cre)2Mpin/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:227671 )

N • no change in parallel fiber synpatic transmission

N • no change in basket/stellate cell synapse-mediated mIPSCs

abnormal synapse morphology ( J:227671 )

• 5% loss of distal climbing-fiber synapse numbers

• 25% decrease in synapse size

decreased excitatory postsynaptic current amplitude ( J:227671 )

• modest decrease (10%) in climbing fiber synapse EPSC amplitude

• no change in paired pulse ratio is detected or synapse elimination

Genotype
MGI:5754949

cn2

• Allelic Composition: Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud; Tg(Pcp2-cre)2Mpin/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

nervous system

nervous system phenotype ( J:227671 )

N • no change in parallel fiber synpatic transmission

N • no change in basket/stellate cell synapse-mediated mIPSCs

decreased excitatory postsynaptic current amplitude ( J:227671 )

• modest decrease (10%) in climbing fiber synapse EPSC amplitude

• no change in paired pulse ratio is detected

Genotype
MGI:5754963

cn3

• Allelic Composition: Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud; Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud; Nlgn3^tm4.1Sud/Y; Tg(Pcp2-cre)2Mpin/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

nervous system

abnormal synaptic bouton morphology ( J:227671 )

• bouton size is reduced

abnormal synapse morphology ( J:227671 )

• 50% decrease in density of climbing fiber synapse on distal Purkinje cell dendrites

• 15% decrease in density of climbing fiber synapse on proximal Purkinje cell dendrites

• inhibitory basket/stellate cell synapses are increased in size, but not number

• 25% decrease in size of climbing fiber synapse on distal and proximal Purkinje cell dendrites

• increase in puncta density as detected by GAD65

• parallel fiber synapse density is not altered

abnormal CNS synaptic transmission ( J:227671 )

• decreased frequency of delayed quantal release events, but not amplitude events

• impaired synaptic transmission at GABAergic synapses

decreased excitatory postsynaptic current amplitude ( J:227671 )

• 40% decrease in amplitude of climbing fiber EPSCs in juvenile and adult mice

• however, no change in kinetics or paired pulse ratio of climbing fiber EPSCs and no alterations in parallel-fiber EPSCs

abnormal inhibitory postsynaptic currents ( J:227671 )

• 80% decrease in IPSC amplitude in Purkinje cells, but no changes in kinetics or paired pulse ratio

abnormal miniature inhibitory postsynaptic currents ( J:227671 )

• 80% decrease in mIPSC frequency and amplitude (45%) in basket/stellate cell synapses

• decrease in decay, but not rise times of mIPSCs

decreased miniature inhibitory postsynaptic current amplitude ( J:227671 )

• 45% decrease in basket/stellate cell synapses

decreased miniature inhibitory postsynaptic current frequency ( J:227671 )

• 80% decrease in basket/stellate cell synapses