All Phenotypes Ppp2ca<tm1.1Jmli> MGI Mouse

abnormal seminiferous tubule morphology ( J:222054 )

• at 4 weeks of age, seminiferous tubules exhibit vacuolation and disrupted arrangement of spermatogonia and primary spermatocytes

• by 8 weeks of age, fewer vacuole-like structures are found, germ cells appear disorganized, and elongated spermatozoa are absent

seminiferous tubule degeneration ( J:222054 )

• at 4 weeks of age, seminiferous tubules exhibit vacuolation

• however, fewer vacuole-like structures are found by 8 weeks of age

small testis ( J:222054 )

• at 4 weeks of age, testes are significantly smaller than wild-type testes

decreased testis weight ( J:222054 )

• at 4 weeks of age, testes are ~20% the weight of control testes

• by 8 weeks of age, testes are ~50% the weight of control testes

abnormal spermatogenesis ( J:222054 )

• by 8 weeks of age, testes are almost completely devoid of mature spermatozoa, as shown by H&E staining of the seminiferous tubules and epididymides

• spermatogenesis is disrupted at all levels, as germ cells are either arranged in a disorderly manner or completely absent

• no normal sperm are observed in a sperm smear collected from P56 epididymides

arrest of male meiosis ( J:222054 )

• at P38, flow cytometric analysis revealed that meiosis is blocked at the diploid stage

• surprisingly, the numbers of haploid and diploid cells are almost identical at P56, indicating that some cells can proceed to the next stage; however, any haploid cells still present at P56 appear abnormal

abnormal cauda epididymis morphology ( J:222054 )

• by 8 weeks of age, cauda epididymides contain some round cells of different sizes and shapes but no normal sperm

male infertility ( J:222054 )

• males fail to produce pups