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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Gpx4tm4Marc
targeted mutation 4, Marcus Conrad
MGI:5769979
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Gpx4tm4Marc/Gpx4tm4Marc involves: 129S6/SvEvTac * C57BL/6J MGI:5803863
ht2
 		Gpx4tm4Marc/Gpx4+ involves: 129S6/SvEvTac * C57BL/6J MGI:5803864


Genotype
MGI:5803863
hm1
Allelic
Composition		 Gpx4tm4Marc/Gpx4tm4Marc
Genetic
Background		 involves: 129S6/SvEvTac * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4tm4Marc mutation (0 available); any Gpx4 mutation (82 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Gpx4tm4Marc/Gpx4tm4Marc embryos show resorptions and severe abnormalities of embryonic and extraembryonic structures

mortality/aging
embryonic lethality between implantation and placentation, complete penetrance ( J:225651 )
• homozygous embryos die at E7.5-E8.5
• an increased number of resorptions and empty decidua, but no viable embryos, are noted at E9.5

embryo
abnormal germ layer development ( J:225651 )
• a clear distinction of the three germinal layers is not possible
abnormal embryonic tissue morphology ( J:225651 )
• severe abnormalities in embryonic structures
abnormal extraembryonic tissue morphology ( J:225651 )
• severe abnormalities in extraembryonic structures

reproductive system
empty decidua capsularis ( J:225651 )
• empty decidua at E9.5




Genotype
MGI:5803864
ht2
Allelic
Composition		 Gpx4tm4Marc/Gpx4+
Genetic
Background		 involves: 129S6/SvEvTac * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4tm4Marc mutation (0 available); any Gpx4 mutation (82 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Severe morphological alterations in the midpiece of developing and mature sperm in Gpx4tm4Marc/Gpx4+ mice

reproductive system
abnormal sperm midpiece morphology ( J:225651 )
• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression particularly in the midpiece of isolated spermatozoa
• many severe morphological defecs are observed in the midpiece and midpiece-principal piece junction of developing and mature sperm
• aberrations include bends mainly at the midpiece-principal piece junction, irregularly aligned mitochondria, and protrusions of outer dense fibers
increased sperm mitochondrial size ( J:225651 )
• late stage spermatids and epididymal spermatozoa exhibit swollen mitochondria surrounding the axoneme
kinked sperm flagellum ( J:225651 )
• bends mainly at the midpiece-principal piece junction
abnormal sperm head morphology ( J:225651 )
• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression in the head of isolated spermatozoa
decreased litter size ( J:225651 )
• heterozygous males yield litter sizes ranging from 2 to 10 pups per litter relative to 1 to 11 pups per litter in wild-type controls
reduced male fertility ( J:225651 )
• male heterozygotes are subfertile in conventional breeding and in vitro fertilization assays
• test breeding of heterozygous males with wild-type C57BL/6J females yielded 11 litters with a total number of 70 offspring whereas breeding of wild-type males yielded 17 litters with a total number of 119 offspring
impaired fertilization ( J:225651 )
• in vitro fertilization assays using heterozygous mutant sperm and wild-type oocytes revealed a significant reduction in the number of zygotes (pro-nuclei stage embryos) and developed blastocysts (16 zygotes; 2 blastocysts) relative to wild-type controls (105 zygotes; 20 blastocysts)
decreased fertilization frequency ( J:225651 )
• in vitro fertilization assays using heterozygous mutant sperm and wild-type oocytes revealed a significant reduction in the number of zygotes (pro-nuclei stage embryos) and developed blastocysts (16 zygotes; 2 blastocysts) relative to wild-type controls (105 zygotes; 20 blastocysts)
abnormal sperm physiology ( J:225651 )
• sperm isolated from the cauda epididymis exhibit higher levels of free reduced thiols than wild-type sperm
abnormal sperm motility ( J:225651 )
• spermatozoa from heterozygous males exhibit a decrease in overall sperm progressivity
• however, total sperm concentration and motility are normal

cellular
abnormal sperm midpiece morphology ( J:225651 )
• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression particularly in the midpiece of isolated spermatozoa
• many severe morphological defecs are observed in the midpiece and midpiece-principal piece junction of developing and mature sperm
• aberrations include bends mainly at the midpiece-principal piece junction, irregularly aligned mitochondria, and protrusions of outer dense fibers
increased sperm mitochondrial size ( J:225651 )
• late stage spermatids and epididymal spermatozoa exhibit swollen mitochondria surrounding the axoneme
kinked sperm flagellum ( J:225651 )
• bends mainly at the midpiece-principal piece junction
abnormal sperm head morphology ( J:225651 )
• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression in the head of isolated spermatozoa
abnormal sperm motility ( J:225651 )
• spermatozoa from heterozygous males exhibit a decrease in overall sperm progressivity
• however, total sperm concentration and motility are normal




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Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory