Phenotypes associated with this allele

• Allele Symbol: Prpf4b^em1(IMPC)J
• Allele Name: endonuclease-mediated mutation 1, Jackson
• Allele ID: MGI:5779705
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prpf4b^em1(IMPC)J/Prpf4b^em1(IMPC)J	C57BL/6NJ-Prpf4b^em1(IMPC)J/J	MGI:6263245

Genotype
MGI:6263245

hm1

• Allelic Composition: Prpf4b^em1(IMPC)J/Prpf4b^em1(IMPC)J
• Genetic Background: C57BL/6NJ-Prpf4b^em1(IMPC)J/J

Data Sources

IMPC Data for Prpf4b

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Prpf4b^em1(IMPC)J/Prpf4b^em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E3.5, appearing as early blastocysts/late morulae, but not at E7.5. Mutants fail to hatch from the zona pellucida with apparent cell death.

embryo

failure of blastocyst formation ( J:279207 )

• in vitro, embryos never reach a morphological blastocyst stage after 3 days in culture

failure of blastocyst to hatch from the zona pellucida ( J:279207 )

• in vitro, embryos fail to hatch from the zona pellucida with apparent cell death after 3 days in culture

embryonic growth retardation ( J:279207 )

• embryos recovered at E3.5 appear as early blastocysts/late morulas

growth/size/body

embryonic growth retardation ( J:279207 )

• embryos recovered at E3.5 appear as early blastocysts/late morulas

mortality/aging

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - JAX

preweaning lethality, complete penetrance ( J:211773 )

IMPC - JAX

embryonic lethality before implantation, complete penetrance ( J:279207 )

• homozygous embryos are recovered at E3.5 (18% versus expected 25%, n=34) and appear to arrest/die prior to blastocyst formation; no embryos or excess resorption sites are observed at E7.5

reproductive system

failure of embryo implantation ( J:279207 )

• in vitro, embryos fail to hatch and never form a normal outgrowth after 3 days in culture