mortality/aging
• homozygous embryos are recovered at slightly lower Mendelian ratios at E3.5 (20% versus expected 25%) and appear to arrest/die prior to blastocyst formation; no embryos or excess resorption sites are observed at E7.5
|
embryo
• embryos fail to reach a morphological blastocyst stage after 3 days in culture
|
• in culture, embryos fail to hatch from the zona pellucida with apparent cell death, never reaching a morphological blastocyst stage
|
• morulas recovered at E3.5 appear to be dying
|
cellular
• morulas recovered at E3.5 appear to be dying
• embryos show no further development with apparent cell death after 3 days in culture
|