Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm1(CAG-Wnt5a,-AcGFP)Skde}
• Allele Name: targeted mutation 1, Sudhansu K Dey
• Allele ID: MGI:5787602
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(CAG-Wnt5a,-AcGFP)Skde/Gt(ROSA)26Sor^+ Pgr^tm2(cre)Lyd/Pgr^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5901730

Genotype
MGI:5901730

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Wnt5a,-AcGFP)Skde}/Gt(ROSA)26Sor⁺; Pgr^tm2(cre)Lyd/Pgr⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:233595 )

♀N • both genes are expressed in ovaria, but ovulation, fertilization, preimplantation embryo development and hormone production are normal in conditional gain-of-function females

abnormal uterus morphology ( J:233595 )

♀ • persistent apicobasal polarity in the luminal epithelium with increased cell heights and retention of glycosylated microvilli on the apical surface

abnormal maternal decidual layer morphology ( J:233595 )

♀ • abnormal primary decidual zone (PDZ) development at 6 dpc

♀ • PDZ was absent in some implantation sites with the embryo entrapped within the intact epithelium at 6 dpc

abnormal embryo attachment ( J:233595 )

♀ • uteri either failed to show or had very weak endometrial vascular permeability at the site of blastocyst attachment at 5 dpc

♀ • unattached blastocysts were recovered from uteri at 5 dpc

♀ • defects in crypt formation

♀ • some blastocysts situated within the primary lumen

abnormal uterine-embryonic axis ( J:233595 )

♀ • inappropriate mesometrial-antimesometrial (M-AM) orientation of implantation sites at 5, 6 and 8 dpc: closer to M pole instead of at AM pole

♀ • ratio of M-AM axis length to anterior-posterior (A-P) axis length is reduced by ~25% resulting in a more spherical morphology at 8 dpc

impaired embryo implantation ( J:233595 )

♀ • uteri either failed to show or had very weak endometrial vascular permeability at the site of blastocyst attachment at 5 dpc

♀ • different sizes of implantation sites at 8 dpc

impaired spacing of implantation sites ( J:233595 )

♀ • implantation sites were often irregularly spaced at 5 and 8 dpc

twin decidual capsule ( J:233595 )

♀ • two embryos closely apposed within one implantation site

reduced female fertility ( J:233595 )

♀ • plug-positive females are either sterile or produce a small litter

♀ • high resorption rates

decreased litter size ( J:233595 )

♀ • in the few fertile mice

embryo

abnormal trophoblast giant cell proliferation ( J:233595 )

♀ • clusters of Prl3d1-positive trophoblast giant cells in implantation sites in lieu of the embryo proper at 10 dpc

abnormal embryo development ( J:233595 )

♀ • increasing numbers of resorption sites on 10 and 12 dpc

♀ • absent or substantially smaller embryos in many implantation sites from 10 dpc

abnormal placenta morphology ( J:233595 )

♀ • in surviving implantation sites at 12 dpc

♀ • disrupted placental development from 10 dpc

abnormal maternal decidual layer morphology ( J:233595 )

♀ • abnormal primary decidual zone (PDZ) development at 6 dpc

♀ • PDZ was absent in some implantation sites with the embryo entrapped within the intact epithelium at 6 dpc

decreased placental labyrinth size ( J:233595 )

♀ • Gcm1-positive syncytiotrophoblasts in the labyrinth failed to elongate, suggesting disrupted secondary branching morphogenesis of placental villi at 12 dpc

cellular

abnormal trophoblast giant cell proliferation ( J:233595 )

♀ • clusters of Prl3d1-positive trophoblast giant cells in implantation sites in lieu of the embryo proper at 10 dpc