Phenotypes associated with this allele

• Allele Symbol: Atp1b1^tm1.1Zboro
• Allele Name: targeted mutation 1.1, Zea Borok
• Allele ID: MGI:5787934
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Aqp5^tm1.1(cre)Pfl/? Atp1b1^tm1.1Zboro/Atp1b1^tm1.1Zboro	129S-Atp1b1^tm1.1Zboro Aqp5^tm1.1(cre)Pfl	MGI:5788090
cn2	Atp1b1^tm1.1Zboro/Atp1b1^tm1.1Zboro Tg(Nkx2-1-cre)2Sand/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6	MGI:5788016
cn3	Atp1b1^tm1.1Zboro/Atp1b1^tm1.1Zboro Tg(Sftpc-cre)1Blh/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:5788091

Genotype
MGI:5788090

cn1

• Allelic Composition: Aqp5^{tm1.1(cre)Pfl}/?; Atp1b1^tm1.1Zboro/Atp1b1^tm1.1Zboro
• Genetic Background: 129S-Atp1b1^tm1.1Zboro Aqp5^{tm1.1(cre)Pfl}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

impaired pulmonary alveolar fluid clearance ( J:233742 )

• 43% reduction in alveolar fluid clearance as compared to controls

respiratory system

respiratory system phenotype ( J:233742 )

N • lung permeability, wet-to-dry lung weight rations and lung morphology are similar to controls

impaired pulmonary alveolar fluid clearance ( J:233742 )

• 43% reduction in alveolar fluid clearance as compared to controls

Genotype
MGI:5788016

cn2

• Allelic Composition: Atp1b1^tm1.1Zboro/Atp1b1^tm1.1Zboro; Tg(Nkx2-1-cre)2Sand/0
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6

mortality/aging

postnatal lethality, complete penetrance ( J:233742 )

• pups die at 2-3 weeks of age

Genotype
MGI:5788091

cn3

• Allelic Composition: Atp1b1^tm1.1Zboro/Atp1b1^tm1.1Zboro; Tg(Sftpc-cre)1Blh/0
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * DBA/2

homeostasis/metabolism

impaired pulmonary alveolar fluid clearance ( J:233742 )

• 78% reduction in alveolar fluid clearance as compared to controls

respiratory system

respiratory system phenotype ( J:233742 )

N • lung permeability, wet-to-dry lung weight ratios and lung morphology are similar to controls

impaired pulmonary alveolar fluid clearance ( J:233742 )

• 78% reduction in alveolar fluid clearance as compared to controls

abnormal respiratory epithelial sodium ion transmembrane transport ( J:233742 )

• IEQ (equivalent short circuit current) is reduced in cultured alveolar epithelial cell monolayers as compared to controls, however, RT (transepithelial electrical resistance) is similar to controls

• cultured alveolar epithelial cell monolayers exhibit lower unidirectional Na+ flux in the apical-to-basolateral direction and unchanged Na+ flux in the basolateral-to-apical direction as compared to controls

• baseline net Na+ absorption is lower than in control monolayers