Analysis Tools|
Allele Symbol Allele Name Allele ID |
Inpp5jtm1.1Cmit targeted mutation 1.1, Christina A Mitchell MGI:5789330 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• homozygotes are viable, fertile and display normal mammary gland development and no overt phenotype at 4 months of age
• no evidence of mammary gland hyperplasia is noted at 1 year of age or spontaneous mammary tumors up to 20 months of age
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• total mammary tumor burden is significantly increased relative to that in control mice carrying the transgene on a wild-type background
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• mice show significantly enhanced primary mammary cancer initiation and tumor progression relative to control mice
• tumors show increased AKT signaling
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• mice show a 4.3-fold reduction in the total number of lung metastases relative to control mice
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• mean volume of the largest mammary tumor per mouse and total tumor volume is significantly higher than that in control mice, and associated with increased cell proliferation as shown by Ki67 staining
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• in transwell assays, epithelial tumor cells established from primary mammary tumors show a 3.5-fold decrease in relative cell migration toward a serum gradient; cell migration defect is partly rescued by treatment with a pan-AKT inhibitor (AktX), suggesting dysregulated AKT signaling
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• total mammary tumor burden is significantly increased relative to that in control mice carrying the transgene on a wild-type background
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• at 45 days of age, mice show a ~5-fold increase in the number of hyperplastic foci in mammary fat pads relative to control mice carrying the transgene on a wild-type background
• total area of mammary gland hyperplasia is increased by 3-fold at 45 days and by 2.4-fold at 70 days of age
• % of proliferating (Ki67+) cells is significantly increased in hyperplastic lesions at 45 and 70 days of age
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• total mammary tumor burden is significantly increased relative to that in control mice carrying the transgene on a wild-type background
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• at 45 days of age, mice show a ~5-fold increase in the number of hyperplastic foci in mammary fat pads relative to control mice carrying the transgene on a wild-type background
• total area of mammary gland hyperplasia is increased by 3-fold at 45 days and by 2.4-fold at 70 days of age
• % of proliferating (Ki67+) cells is significantly increased in hyperplastic lesions at 45 and 70 days of age
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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