Phenotypes associated with this allele

• Allele Symbol: Olfm4^{tm2.1(EGFP/cre/ERT2)Cle}
• Allele Name: targeted mutation 2.1, Hans Clevers
• Allele ID: MGI:5805245
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Olfm4^tm2.1(EGFP/cre/ERT2)Cle/Olfm4^+	involves: 129P2/OlaHsd * C57BL/6	MGI:6401287
cn2	Olfm4^tm2.1(EGFP/cre/ERT2)Cle/Olfm4^+ Znhit1^tm1.1Nju/Znhit1^tm1.1Nju	involves: 129P2/OlaHsd * C57BL/6	MGI:6360246

Genotype
MGI:6401287

ht1

• Allelic Composition: Olfm4^{tm2.1(EGFP/cre/ERT2)Cle}/Olfm4⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

digestive/alimentary phenotype ( J:285672 )

• mice exhibit normal intestinal morphology and physiology under basal conditions and normal response to irradiation

abnormal intestinal epithelium physiology ( J:285672 )

• intestinal stem cells treated with tamoxifen exhibit impaired organoid formation compared with control cells

Genotype
MGI:6360246

cn2

• Allelic Composition: Olfm4^{tm2.1(EGFP/cre/ERT2)Cle}/Olfm4⁺; Znhit1^tm1.1Nju/Znhit1^tm1.1Nju
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

premature death ( J:276456 )

• all mice die by 18 days post tamoxifen treatment

digestive/alimentary system

abnormal intestine development ( J:276456 )

• postnatal depletion of intestinal stem cells in tamoxifen-treated mice

• however, embryonic development of Lgr5+ progenitor cells is normal