Phenotypes associated with this allele

• Allele Symbol: Adk^tm2Bois
• Allele Name: targeted mutation 2, Detlev Boison
• Allele ID: MGI:5816711
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Adk^tm2Bois/Adk^tm2Bois Adora1^tm1Bbf/Adora1^tm1Bbf Tg(Nes-cre)1Kln/0	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5816716
cn2	Adk^tm2Bois/Adk^tm2Bois Adora2a^tm1Jfc/Adora2a^tm1Jfc Tg(Nes-cre)1Kln/0	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:5816717
cn3	Adk^tm2Bois/Adk^tm2Bois Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL	MGI:5816712

Genotype
MGI:5816716

cn1

• Allelic Composition: Adk^tm2Bois/Adk^tm2Bois; Adora1^tm1Bbf/Adora1^tm1Bbf; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

environmentally induced seizures ( J:237189 )

♂ • mice exhibit increased susceptibility to stress-induced (placement in novel environment) seizures

mortality/aging

premature death ( J:237189 )

♂ • increase in mortality

nervous system

environmentally induced seizures ( J:237189 )

♂ • mice exhibit increased susceptibility to stress-induced (placement in novel environment) seizures

Genotype
MGI:5816717

cn2

• Allelic Composition: Adk^tm2Bois/Adk^tm2Bois; Adora2a^tm1Jfc/Adora2a^tm1Jfc; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

nervous system

decreased susceptibility to induction of seizure by inducing agent ( J:237189 )

♂ • mice exhibit a resistance to stress-induced (placement in novel environment) seizures

behavior/neurological

behavior/neurological phenotype ( J:237189 )

♂N • mice exhibit normal associative learning and contextual learning

decreased susceptibility to induction of seizure by inducing agent ( J:237189 )

♂ • mice exhibit a resistance to stress-induced (placement in novel environment) seizures

Genotype
MGI:5816712

cn3

• Allelic Composition: Adk^tm2Bois/Adk^tm2Bois; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: C57BL/6 * SJL

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:237189 )

♂ • mice show increased susceptibility to induced seizures with the adenosine A1 receptor antagonist DPCPX, and a single high dose of DPCPX consistently triggers lethal status epilepticus in mutants but not controls

♂ • pretreatment with antagonists to adenosine A2a receptor (SCH58261) and TrkB (Ana-12) significantly extend the survival time after DPCPX-induced seizures

abnormal associative learning ( J:237189 )

♂ • associative learning in the conditioned freezing paradigm is decreased during conditioned stimulus 2

♂ • however, the percentage time freezing during conditioned stimulus 3 and 4 is increased compared with baseline conditioned stimulus 1 freezing indicating that mice show a general ability to learn

impaired contextual conditioning behavior ( J:237189 )

♂ • mice show a deficit in contextual memory with freezing rates comparable to baseline

♂ • however, mutants show no differences from controls in the elevated plus maze, acoustic startle response, or spatial working memory and the response to foot shock is normal

environmentally induced seizures ( J:237189 )

♂ • from 2 months of age, mice exhibit increased susceptibility to stress-induced seizures (placement in novel environment) which are characterized by tonic-clonic convulsions

♂ • by 3 months of age, 44% of mutants react with a seizure when placed into a novel environment, by 4 months, seizure incidence is 89% and by 6 months, 100%

♂ • the probability of an evoked seizure response rises from 7.4% at 2 months to 77% at 6 months

convulsive seizures ( J:237189 )

♂ • EEG recordings indicate spontaneous seizures with average seizure rate of 0.85 seizures per day and average duration of 43.6 +/- 7.4 seconds

nervous system

increased susceptibility to pharmacologically induced seizures ( J:237189 )

♂ • mice show increased susceptibility to induced seizures with the adenosine A1 receptor antagonist DPCPX, and a single high dose of DPCPX consistently triggers lethal status epilepticus in mutants but not controls

♂ • pretreatment with antagonists to adenosine A2a receptor (SCH58261) and TrkB (Ana-12) significantly extend the survival time after DPCPX-induced seizures

environmentally induced seizures ( J:237189 )

♂ • from 2 months of age, mice exhibit increased susceptibility to stress-induced seizures (placement in novel environment) which are characterized by tonic-clonic convulsions

♂ • by 3 months of age, 44% of mutants react with a seizure when placed into a novel environment, by 4 months, seizure incidence is 89% and by 6 months, 100%

♂ • the probability of an evoked seizure response rises from 7.4% at 2 months to 77% at 6 months

convulsive seizures ( J:237189 )

♂ • EEG recordings indicate spontaneous seizures with average seizure rate of 0.85 seizures per day and average duration of 43.6 +/- 7.4 seconds

abnormal synaptic plasticity ( J:237189 )

♂ • mice have increased adenosine A2a receptor-mediated synaptic plasticity

abnormal CNS synaptic transmission ( J:237189 )

♂ • mice have increased levels of synaptic adenosine

♂ • treatment of hippocampal slices from epileptic mutants with the adenosine A1 receptor antagonist DPCPX results in a higher disinhibition of synaptic transmission than controls

abnormal excitatory postsynaptic potential ( J:237189 )

♂ • maximum fEPSP slope is higher in mutant hippocampal slices than in controls

enhanced long-term potentiation ( J:237189 )

♂ • hippocampal slices show an increase in theta-burst-induced LTP magnitude

♂ • treatment with the adenosine A2a receptor-selective antagonist SCH58261resverses the LTP magnitude

♂ • treatment with the tyrosine kinase inhibitor K25a abolishes the increased LTP magnitude