Phenotypes associated with this allele

• Allele Symbol: Tg(Col1a1-Dkk1)1Hmk
• Allele Name: transgene insertion 1, Henry M Kronenberg
• Allele ID: MGI:5818202
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Col1a1-Dkk1)1Hmk/?	B6.FVB-Tg(Col1a1-Dkk1)1Hmk	MGI:5818857

Genotype
MGI:5818857

tg1

• Allelic Composition: Tg(Col1a1-Dkk1)1Hmk/?
• Genetic Background: B6.FVB-Tg(Col1a1-Dkk1)1Hmk

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell cycle ( J:238121 )

• increased cell cycling as determined by increased sensitivity to 5-FU

• lag in the ability of bone marrow to replace depleted neutrophil pool, and to a lesser extent, white blood cells following 5-FU treatment

• LK+ S+ cell population is depleted of hematopoietic stem cells in G0 fraction

impaired osteoblast differentiation ( J:158620 )

• osteoblast number and activity are decreased in tibia from 12 week old mice

hematopoietic system

abnormal definitive hematopoiesis ( J:238121 )

• defect in multilineage and myeloid colony formation in bone marrow

• hematopoietic stem cells are are unable to retain a population of quiescent G0 cells

increased common lymphocyte progenitor cell number ( J:238121 )

• slight but significant increase in common lymphoid progenitors in bone marrow

increased osteoclast cell number ( J:158620 )

• increase in number of multinucleated osteoclasts in primary spongiosa of tibia from 6 week old mice

• however, there is no increase in osteoclast number or surface in secondary spongiosa

abnormal osteoclast physiology ( J:158620 )

• on a low calcium diet osteoclast activity in primary spongiosa is increased, in contrast to a decrease in wild-type mice

abnormal hematopoietic stem cell physiology ( J:238121 )

• hematopoietic stem cells are are unable to retain a population of quiescent G0 cells

• LK+ S+ cell population is depleted of hematopoietic stem cells in G0 fraction

homeostasis/metabolism

insensitivity to parathyroid hormone ( J:158620 )

• continuous infusion of parathyroid hormone causes loss of metaphyseal trabecular bone rather than increased metaphyseal trabecular bone formation

immune system

increased osteoclast cell number ( J:158620 )

• increase in number of multinucleated osteoclasts in primary spongiosa of tibia from 6 week old mice

• however, there is no increase in osteoclast number or surface in secondary spongiosa

abnormal osteoclast physiology ( J:158620 )

• on a low calcium diet osteoclast activity in primary spongiosa is increased, in contrast to a decrease in wild-type mice

abnormal response to transplant ( J:238121 )

• defect in bone marrow reconstitution potential of hematopoietic stem cells in serial transplantation or following secondary transplantation of wild type donor cells

skeleton

impaired osteoblast differentiation ( J:158620 )

• osteoblast number and activity are decreased in tibia from 12 week old mice

increased osteoclast cell number ( J:158620 )

• increase in number of multinucleated osteoclasts in primary spongiosa of tibia from 6 week old mice

• however, there is no increase in osteoclast number or surface in secondary spongiosa

decreased bone mineral density ( J:158620 )

• mild osteopenia

decreased trabecular bone volume ( J:158620 , J:238121 )

• bone volume is decreased 40% in femurs and 25% in vertebrae (J:158620)

• trabecular bone volume is reduced by 20% (J:238121)

• cortical bone volume is not affected (J:238121)

decreased osteoblast cell number ( J:158620 )

• osteoblast number and activity are decreased in tibia from 12 week old mice

decreased bone trabecula number ( J:158620 )

• decrease in trabecular number, trabecular connectivity density and structure model index

increased bone trabecular spacing ( J:158620 )

• increase in trabecular separation

decreased trabecular bone connectivity density ( J:158620 )

• decrease in trabecular number, trabecular connectivity density and structure model index

abnormal skeleton physiology ( J:158620 )

• on a low calcium diet, mice fail to develop the peritrabecular stromal cell (osteitis fibrosis) and woven bone response expected in this induced model of sustained secondary hyperpararthyroidism

abnormal osteoclast physiology ( J:158620 )

• on a low calcium diet osteoclast activity in primary spongiosa is increased, in contrast to a decrease in wild-type mice

abnormal bone mineralization ( J:158620 )

• decrease in mineral apposition rate and a 60% decrease in bone formation rate

• substantial reduction of mineralized trabecular in the metaphyseal trabecular regions

decreased bone ossification ( J:158620 )

• decrease in mineral apposition rate and a 60% decrease in bone formation rate

• increased percentage of cells undergoing apoptosis

decreased bone strength ( J:158620 )

• on a low calcium diet, some mice exhibit tibial fractures