Phenotypes associated with this allele

• Allele Symbol: Vit^tm1Koth
• Allele Name: targeted mutation 1, Kunimasa Ohta
• Allele ID: MGI:5823247
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vit^tm1Koth/Vit^tm1Koth	B6J.Cg-Vit^tm1Koth	MGI:5823836

Genotype
MGI:5823836

hm1

• Allelic Composition: Vit^tm1Koth/Vit^tm1Koth
• Genetic Background: B6J.Cg-Vit^tm1Koth

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal neuron differentiation ( J:237454 )

• at E13.5, mice show altered differentiation of progenitor cells in the spinal cord central canal area

abnormal neural tube morphology ( J:237454 )

• embryos exhibit abnormal dorso-ventral patterning and reduced size of the neural tube

abnormal spinal cord ependymal layer morphology ( J:237454 )

• in culture, secondary neurospheres derived from P0 spinal cords show altered distribution of ependymal proliferation markers, with Cyclin D2 and Ki67 expression observed throughout the neurosphere area instead of the outer circumference as in wild-type spheres

abnormal neuronal stem cell morphology ( J:237454 )

• at E13.5, immunostaining revealed reduced expression of nestin (neural stem/progenitor cell marker) and GFAP (neural stem cell marker) in the spinal cord

decreased motor neuron number ( J:237454 )

• at E13.5, Isl1/2-positive motor neuron cells are significantly decreased in the spinal cord

decreased neuronal precursor cell number ( J:237454 )

• at E13.5, immunostaining revealed reduced expression of nestin (neural stem/progenitor cell marker) in the central canal of the spinal cord

• at E12.5, expression of Pax6 (neural progenitor cell marker) is reduced in the central region (p0-p2) of the spinal cord

abnormal spinal cord interneuron morphology ( J:237454 )

• at E13.5, the total number of Isl1/2-positive cells (motoneuron and interneuron markers) are significantly reduced in the spinal cord

abnormal spinal cord central canal morphology ( J:237454 )

• at E13.5, mice show decreased cell proliferation and altered cell differentiation in the spinal cord central canal area

• however, blood vessel formation around the central canal is normal

decreased spinal cord size ( J:237454 )

• at E16.5, the width and length of the spinal cord is significantly reduced at the thoracic level

• BrdU-positive cells are significantly reduced at E13.5 and E16.5, indicating decreased cell proliferation

• after 5 days in culture, the diameter, but not number, of neurospheres derived from the P6 spinal cord thoracic region is significantly reduced relative to that in wild-type spheres

embryo

abnormal neural tube morphology ( J:237454 )

• embryos exhibit abnormal dorso-ventral patterning and reduced size of the neural tube

cellular

abnormal neuron differentiation ( J:237454 )

• at E13.5, mice show altered differentiation of progenitor cells in the spinal cord central canal area