Phenotypes associated with this allele

• Allele Symbol: Lama3^tm1.1Grsb
• Allele Name: targeted mutation 1.1, G R Scott Budinger
• Allele ID: MGI:5825195
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Lama3^tm1.1Grsb/Lama3^tm1.1Grsb	involves: C57BL/6	MGI:5883495

Genotype
MGI:5883495

cn1

• Allelic Composition: Lama3^tm1.1Grsb/Lama3^tm1.1Grsb
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

respiratory system

increased alveolar macrophage number ( J:235712 )

• Ad-Cre-treated mice exhibit increased numbers of alveolar macrophages relative to Ad-Null-treated mice

lung inflammation ( J:235712 )

• bronchoalveolar lavage (BAL) fluid from Ad-Cre-treated mice contains more inflammatory cells (alveolar macrophages, interstitial macrophages and neutrophils) than that from Ad-Null-treated mice, as revealed by dispase digestion of the lungs

• Ad-Cre-treated mice show a small, but significant, increase in the BAL fluid levels of the proinflammatory cytokines TNF and CCL2 relative to Ad-Null-treated mice

• following injurious mechanical ventilation, the increase in BAL cell count is markedly higher in Ad-Cre-treated mice relative to Ad-Null-treated mice

abnormal alveolar macrophage physiology ( J:235712 )

• at baseline, alveolar macrophages from Ad-Cre-treated mice express higher levels of activation markers on their surface than those from Ad-Null-treated mice

abnormal lung hysteresivity ( J:235712 )

• at 60 days after intratracheal treatment of adult mice with Ad-Cre, mice show a significant increase in lung hysteresivity relative to control mice treated Ad-Null

decreased airway resistance ( J:235712 )

• at 60 days after intratracheal treatment of adult mice with Ad-Cre, mice show a significant decrease in Newtonian resistance (a measure of central airways resistance) relative to control mice treated with Ad-Null

• however, baseline lung histology, gas exchange, and other lung mechanics including pressure volume curves and key measures of lung compliance remain normal

homeostasis/metabolism

abnormal protein level ( J:235712 )

• following exposure to mechanical ventilation induced lung injury, Ad-Cre-treated mice exhibit a lower concentration of protein in the BAL fluid (a measure of the permeability of alveolar-capillary barrier to macromolecules) than Ad-Null-treated mice

increased collagen level ( J:235712 )

• Ad-Cre-treated mice exhibit a significant increase in type I collagen but no change in type IV collagen in the lungs relative to Ad-Null-treated mice

abnormal chemokine level ( J:235712 )

• Ad-Cre-treated mice show a small, but significant, increase in the BAL concentrations of chemokine (C-C motif) ligand 2 (CCL2) relative to Ad-Null-treated mice

abnormal tumor necrosis factor level ( J:235712 )

• Ad-Cre-treated mice show a small, but significant, increase in the BAL concentrations of TNF relative to Ad-Null-treated mice

abnormal susceptibility to injury induced morbidity/mortality ( J:235712 )

• following exposure to mechanical ventilation induced lung injury (tidal volume of 35 ml per kg of body weight for 2 hours), Ad-Cre-treated mice exhibit significantly less mortality than Ad-Null-treated mice

decreased susceptibility to injury ( J:235712 )

• following exposure to mechanical ventilation induced lung injury (tidal volume of 35 ml per kg of body weight for 2 hours), Ad-Cre-treated mice exhibit less severe injury, as shown by improved lung compliance, decreased interstitial edema and hemorrhage, a smaller increase in alveolar capillary permeability, and increased survival relative to Ad-Null-treated mice

immune system

increased alveolar macrophage number ( J:235712 )

• Ad-Cre-treated mice exhibit increased numbers of alveolar macrophages relative to Ad-Null-treated mice

abnormal chemokine level ( J:235712 )

• Ad-Cre-treated mice show a small, but significant, increase in the BAL concentrations of chemokine (C-C motif) ligand 2 (CCL2) relative to Ad-Null-treated mice

abnormal tumor necrosis factor level ( J:235712 )

• Ad-Cre-treated mice show a small, but significant, increase in the BAL concentrations of TNF relative to Ad-Null-treated mice

lung inflammation ( J:235712 )

• bronchoalveolar lavage (BAL) fluid from Ad-Cre-treated mice contains more inflammatory cells (alveolar macrophages, interstitial macrophages and neutrophils) than that from Ad-Null-treated mice, as revealed by dispase digestion of the lungs

• Ad-Cre-treated mice show a small, but significant, increase in the BAL fluid levels of the proinflammatory cytokines TNF and CCL2 relative to Ad-Null-treated mice

• following injurious mechanical ventilation, the increase in BAL cell count is markedly higher in Ad-Cre-treated mice relative to Ad-Null-treated mice

mortality/aging

abnormal susceptibility to injury induced morbidity/mortality ( J:235712 )

• following exposure to mechanical ventilation induced lung injury (tidal volume of 35 ml per kg of body weight for 2 hours), Ad-Cre-treated mice exhibit significantly less mortality than Ad-Null-treated mice

hematopoietic system

increased alveolar macrophage number ( J:235712 )

• Ad-Cre-treated mice exhibit increased numbers of alveolar macrophages relative to Ad-Null-treated mice