Phenotypes associated with this allele

• Allele Symbol: Ankle1^{tm1.2(KOMP)Wtsi}
• Allele Name: targeted mutation 1.2, Wellcome Trust Sanger Institute
• Allele ID: MGI:5827623
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ankle1^tm1.2(KOMP)Wtsi/Ankle1^tm1.2(KOMP)Wtsi	involves: C57BL/6J * C57BL/6N	MGI:5827624

Genotype
MGI:5827624

hm1

• Allelic Composition: Ankle1^{tm1.2(KOMP)Wtsi}/Ankle1^{tm1.2(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6J * C57BL/6N
• Cell Lines: EPD0323_2_B12

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, incomplete penetrance ( J:235782 )

• heterozygous matings yield homozygotes at a reduced birth Mendelian ratio (17% vs expected 25%) with no detectable abnormalities in E13.5 homozygous embryos

reproductive system

reproductive system phenotype ( J:235782 )

N • pairings of homozygotes yield normal litter sizes of up to 12 pups

hematopoietic system

hematopoietic system phenotype ( J:235782 )

N • homozygotes exhibit normal hematopoiesis with no detectable defects in hematopoietic progenitor cells, myeloid and lymphoid progenitors or B and T cell development in the bone marrow, spleen and thymus

N • embryonic stress erythropoiesis in liver and adult erythropoiesis in bone marrow and spleen appear unaffected

cellular

cellular phenotype ( J:235782 )

N • in culture, primary mouse embryonic fibroblasts (MEFs) derived from E13.5 embryos show normal growth curves in the absence of any treatment, and exhibit normal survival rates after chronic exposure to various DNA-damage inducing agents or 72 h after single exposure to increasing doses of UV-light relative to similarly treated wild-type controls

N • upon Blm (Bloom syndrome, RecQ helicase-like) depletion by siRNA, immortalized MEFs exhibit sister chromatid exchange rates similar to those seen in wild-type MEFs