immune system
• following LPS treatment (1 ug/ml) for 24 h, bone marrow-derived macrophages (BMDMs) show increased accumulation of CCL2 in the Golgi relative to LPS-treated wild-type cells, indicating altered trafficking of specific chemokines
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• following LPS treatment for 24 h, production of several chemokines (CCL3, CCL5, and CXCL2) and of ICAM1 (CD54) is reduced in BMDM lysates relative to that in LPS-treated wild-type controls
• after LPS treatment, the amount of secreted CCL2 is significantly decreased in cell culture supernatants at all time points, as measured by ELISA
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• mice exhibit impaired recruitment of peripheral macrophages in response to i.p. injections of LPS or live bacteria, suggesting an altered innate immune response
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• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b+F4/80dim peritoneal macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), the % of recruited CD11b+F4/80dim macrophages is significantly reduced relative to that in wild-type controls
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• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls
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hematopoietic system
• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b+F4/80dim peritoneal macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), the % of recruited CD11b+F4/80dim macrophages is significantly reduced relative to that in wild-type controls
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• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls
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cellular
• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b+F4/80dim peritoneal macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), the % of recruited CD11b+F4/80dim macrophages is significantly reduced relative to that in wild-type controls
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• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls
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homeostasis/metabolism
• following LPS treatment (1 ug/ml) for 24 h, bone marrow-derived macrophages (BMDMs) show increased accumulation of CCL2 in the Golgi relative to LPS-treated wild-type cells, indicating altered trafficking of specific chemokines
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