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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Mcoln2tm1e.1(KOMP)Wtsi
targeted mutation 1e.1, Wellcome Trust Sanger Institute
MGI:5881907
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Mcoln2tm1e.1(KOMP)Wtsi/Mcoln2tm1e.1(KOMP)Wtsi involves: C57BL/6J * C57BL/6N MGI:5904844


Genotype
MGI:5904844
hm1
Allelic
Composition
Mcoln2tm1e.1(KOMP)Wtsi/Mcoln2tm1e.1(KOMP)Wtsi
Genetic
Background
involves: C57BL/6J * C57BL/6N
Cell Lines EPD0300_5_B06
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mcoln2tm1e.1(KOMP)Wtsi mutation (0 available); any Mcoln2 mutation (36 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• following LPS treatment (1 ug/ml) for 24 h, bone marrow-derived macrophages (BMDMs) show increased accumulation of CCL2 in the Golgi relative to LPS-treated wild-type cells, indicating altered trafficking of specific chemokines
• following LPS treatment for 24 h, production of several chemokines (CCL3, CCL5, and CXCL2) and of ICAM1 (CD54) is reduced in BMDM lysates relative to that in LPS-treated wild-type controls
• after LPS treatment, the amount of secreted CCL2 is significantly decreased in cell culture supernatants at all time points, as measured by ELISA
• mice exhibit impaired recruitment of peripheral macrophages in response to i.p. injections of LPS or live bacteria, suggesting an altered innate immune response
• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b+F4/80dim peritoneal macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), the % of recruited CD11b+F4/80dim macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls

hematopoietic system
• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b+F4/80dim peritoneal macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), the % of recruited CD11b+F4/80dim macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls

cellular
• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b+F4/80dim peritoneal macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), the % of recruited CD11b+F4/80dim macrophages is significantly reduced relative to that in wild-type controls
• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls
• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 107 CFU), migration of Ly6G+CD16+ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls

homeostasis/metabolism
• following LPS treatment (1 ug/ml) for 24 h, bone marrow-derived macrophages (BMDMs) show increased accumulation of CCL2 in the Golgi relative to LPS-treated wild-type cells, indicating altered trafficking of specific chemokines





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last database update
11/05/2024
MGI 6.24
The Jackson Laboratory