Phenotypes associated with this allele

• Allele Symbol: Cpne6^tm1.1Rueg
• Allele Name: targeted mutation 1.1, Markus Ruegg
• Allele ID: MGI:5882517
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cpne6^tm1.1Rueg/Cpne6^tm1.1Rueg	B6.Cg-Cpne6^tm1.1Rueg	MGI:6279162
hm2	Cpne6^tm1.1Rueg/Cpne6^tm1.1Rueg	involves: 129 * 129S1/Sv * C57BL/6	MGI:6279160

Genotype
MGI:6279162

hm1

• Allelic Composition: Cpne6^tm1.1Rueg/Cpne6^tm1.1Rueg
• Genetic Background: B6.Cg-Cpne6^tm1.1Rueg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:239922 )

N • adult male homozygotes exhibit normal open field behavior and foot-shock sensitivity

impaired contextual conditioning behavior ( J:239922 )

• in a fear-conditioning paradigm, adult male homozygotes exhibit a significant deficit in learning and context-dependent memory relative to wild-type controls

• however, no deficits are observed in amygdala-dependent cued memory

Genotype
MGI:6279160

hm2

• Allelic Composition: Cpne6^tm1.1Rueg/Cpne6^tm1.1Rueg
• Genetic Background: involves: 129 * 129S1/Sv * C57BL/6

nervous system

nervous system phenotype ( J:239922 )

N • adult homozygotes exhibit normal levels of postsynaptic density proteins and calcium signaling targets and normal brain weight and morphology, with no significant changes in spine density and morphology or frequency and amplitude of miniature excitatory and inhibitory postsynaptic currents in hippocampal CA1 neurons relative to wild-type controls

abnormal dendritic spine morphology ( J:239922 )

• after 40 min of chemical LTP induction, DIV21 (day in vitro 21) cultured hippocampal neurons fail to exhibit a significant increase in postsynaptic spine head width, unlike wild-type hippocampal neurons

impaired synaptic plasticity ( J:239922 )

• after 40 min of chemical LTP induction, DIV21 cultured hippocampal neurons fail to exhibit a significant increase in postsynaptic spine head width, indicating absence of spine structural plasticity

abnormal excitatory postsynaptic potential ( J:239922 )

• after high-frequency stimulation of Schaffer collaterals, the initial increase in excitatory postsynaptic current in CA1 pyramidal neurons is normal but the fEPSP slope returns to baseline within 60 min of LTP induction, unlike in wild-type controls

• however, the input-output ratio of Schaffer collateral-CA1 connections and paired-pulse ratio (fEPSP₂ slope to fEPSP₁ slope) are normal

reduced long-term potentiation ( J:239922 )

• adult homozygotes show a deficit in hippocampal LTP maintenance, as measured by fEPSPs in CA1 pyramidal neurons by high-frequency stimulation of Schaffer collaterals in acute hippocampal slices

• short-term treatment of acute hippocampal slices with jasplakinolid (an actin stabilizer) fully restores the LTP deficit in CA1 neurons to wild-type levels

• NMDA receptor-dependent long term depression (LTD), triggered by low-frequency stimulation at 1Hz, is normal