Phenotypes associated with this allele

• Allele Symbol: Smc5^{tm1c(KOMP)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:5882565
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(cre/ERT2)Tyj/0 Smc5^tm1c(KOMP)Wtsi/Smc5^tm1d(KOMP)Wtsi	involves: 129S4/SvJae * C57BL/6N * CBA	MGI:5902337

Genotype
MGI:5902337

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/0; Smc5^{tm1c(KOMP)Wtsi}/Smc5^{tm1d(KOMP)Wtsi}
• Genetic Background: involves: 129S4/SvJae * C57BL/6N * CBA
• Cell Lines: EPD0395_1_F05

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

cellular phenotype ( J:240155 )

N • in embryonic stem cells after after 5 and 8 days of tamoxifen exposure

abnormal cell cycle ( J:240155 )

• 10% decrease in number of cells in S phase

• 10% increase in number of cells in G2 phase

abnormal mitosis ( J:240155 )

• mitotic delay

• accumulation of cells in G2 phase

• decreased condensin accumulation at pericentromeric regions in prometaphase cells

• increased condensin signal along chromosome arms in prometaphase cells

• increase in number of polyploid cells

• formation of chromosomal bridges

• lagging chromosomes

• reduced Plk1 enrichment at pericentromeric regions

• persistent localization of Aurkb along chromosome arms

abnormal mitotic spindle assembly checkpoint ( J:240155 )

• reduced Mad2l1 enrichment at pericentromeric regions

abnormal mitotic spindle morphology ( J:240155 )

increased mitotic index ( J:240155 )

• 10% increase in number of cells in G2 phase

mitotic nondisjunction ( J:240155 )

• 80% of cells could not complete accurate chromosome segregation

increased cell death ( J:240155 )

• acetylation at Lys379 of genotoxic stress marker Trp53, indicating stimulation of transcriptional activity

• cleavage of Parp1, indicating an apoptotic signal

abnormal cell differentiation ( J:240155 )

• shift in the pluripotent state by induction of differentiation marker expression