Phenotypes associated with this allele

• Allele Symbol: Tg(Afp-ADA)#Xiay
• Allele Name: transgene insertion, Yang Xia
• Allele ID: MGI:5897389
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Ada^tm1Mw/Ada^tm1Mw Adora2b^tm1Dgen/Adora2b^tm1Dgen Tg(Afp-ADA)#Xiay/0	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C3H/HeJ * C57BL/6J	MGI:5897419
cx2	Ada^tm1Mw/Ada^tm1Mw Tg(Afp-ADA)#Xiay/0	involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J	MGI:5897391

Genotype
MGI:5897419

cx1

• Allelic Composition: Ada^tm1Mw/Ada^tm1Mw; Adora2b^tm1Dgen/Adora2b^tm1Dgen; Tg(Afp-ADA)#Xiay/0
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C3H/HeJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:234143 )

♀N • preeclampsia features seen in Ada^tm1Mw/Ada^tm1Mw Tg(Afp-ADA)#Xiay/0 dams, including hypertension are ameliorated

renal/urinary system

renal/urinary system phenotype ( J:234143 )

♀N • preeclampsia features seen in Ada^tm1Mw/Ada^tm1Mw Tg(Afp-ADA)#Xiay/0 dams dams, including proteinuria and renal changes are ameliorated

embryo

abnormal placenta morphology ( J:234143 )

♀ • mice exhibit an increase in placental adenosine levels

♀ • however, placental weight is increased and placental vasculature is more organized and uniform compared to Ada^tm1Mw/Ada^tm1Mw Tg(Afp-ADA)#Xiay/0 mice

Genotype
MGI:5897391

cx2

• Allelic Composition: Ada^tm1Mw/Ada^tm1Mw; Tg(Afp-ADA)#Xiay/0
• Genetic Background: involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J

mortality/aging

postnatal lethality, complete penetrance ( J:234143 )

• mice die by 3 weeks of age because hepatic production of ADA subsides during the first week following birth

• however, treatment with adenosine deaminase enzyme replacement therapy prevents this lethality and the phenotypes described are with mice that have been treated with the replacement therapy from birth

cellular

maternal effect ( J:234143 )

• fetuses from mutant pregnant females are smaller and weigh less

cardiovascular system

abnormal placenta vasculature ( J:234143 )

♀ • pregnant mice show impaired placental vasculature and reduced CD31+ vessels in the placenta

hypertension ( J:234143 )

♀ • dams develop preeclampsia

increased systemic arterial systolic blood pressure ( J:234143 )

♀ • dams show an increase in mean systolic blood pressure late in pregnancy beginning on E15.5, which remains elevated through E17.5 and returns to normal by day 5 postpartum

♀ • mean arterial pressure is elevated on E18.5 in dams

embryo

abnormal placenta morphology ( J:234143 )

♀ • pregnant mice exhibit elevated placental adenosine levels at E12.5 that remain elevated through E18.5

abnormal placenta vasculature ( J:234143 )

♀ • pregnant mice show impaired placental vasculature and reduced CD31+ vessels in the placenta

small placenta ( J:234143 )

♀

decreased placenta weight ( J:234143 )

♀

homeostasis/metabolism

increased urine protein level ( J:234143 )

♀ • urinary protein (ratio of albumin/creatinine) is elevated on E18.5 but not on E12.5, and this proteinuria is reduced to normal levels by postpartum day 7

renal/urinary system

increased urine protein level ( J:234143 )

♀ • urinary protein (ratio of albumin/creatinine) is elevated on E18.5 but not on E12.5, and this proteinuria is reduced to normal levels by postpartum day 7

abnormal renal glomerulus morphology ( J:234143 )

♀ • swollen glomeruli with narrowed capillary and Bowmans spaces in pregnant mice

♀ • pregnant mice show glomerular endotheliosis

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
pre-eclampsia		DOID:10591	OMIM:189800 OMIM:609402 OMIM:609403 OMIM:609404 OMIM:614592	J:234143