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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Braftm1Tumg
targeted mutation 1, Yoko Aoki
MGI:5902220
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
involves: BALB/c * C57BL * C57BL/6J * DBA MGI:6164158
cn2
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
involves: C57BL * C57BL/6J * DBA MGI:5902221
cn3
Braftm1Tumg/Braftm1Tumg
Tg(CAG-cre)2Osb/0
involves: C57BL * C57BL/6J * DBA * ICR MGI:6164160
cn4
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
involves: C57BL * C57BL/6J * DBA * ICR MGI:6164161


Genotype
MGI:6164158
cn1
Allelic
Composition
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
Genetic
Background
involves: BALB/c * C57BL * C57BL/6J * DBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Tumg mutation (0 available); any Braf mutation (60 available)
Tg(CAG-cre)2Osb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• a few mice survive to adulthood, although all mice die within 24 weeks
• about 50% of mice die at P5 and 90% at weaning (P28)

growth/size/body
• mice exhibit higher heart weight to body weight ratio at P5, indicating cardiomegaly

cardiovascular system
• 5 of 6 mice exhibit a large secundum atrial septal defect (ASDII), while 1 of 6 mice exhibit a small ASDII or patent foramen ovale
• however, cardiac valve defects and apparent abnormalities in other tissues are not seen
• 1 of 6 mice exhibit a small ASDII or patent foramen ovale
• mice exhibit higher heart weight to body weight ratio at P5, indicating cardiomegaly

integument

liver/biliary system
• 36% of mice show liver necrosis at P3

reproductive system
• females are infertile
• however, males are fertile




Genotype
MGI:5902221
cn2
Allelic
Composition
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
Genetic
Background
involves: C57BL * C57BL/6J * DBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Tumg mutation (0 available); any Braf mutation (60 available)
Tg(CAG-cre)2Osb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• 93% of hearts at E16.5 show various defects
• 3 of 14 embryos show hypoplasia of the coronary arteries
• E14.5 hearts show an increase in density of trabeculae (hypertrabeculation)
• 4 of 14 embryos exhibit a thickened trabecular layer and thinned compact layer in the left, right, or combined myocardium
• 4 of 14 embryos exhibit a thickened trabecular layer and thinned compact layer in the left, right, or combined myocardium
• hearts exhibit enhanced cell proliferation
• at E13.5, the interventricular septum and myocardium show enhanced cell proliferation
• at E16.5, the pulmonary valves and the interventricular septum in fetuses with ventricular septal defect, show enhanced cell proliferation
• 2 of 14 embryos exhibit abnormal endocardial cushion
• 9 of 14 embryos show hypertrophy of mitral valves
• co-treatment with PD0325901 and an inhibitor of histone H3K27 demethylase UTX, GSK-J4, ameliorates enlarged mitral valves, however, no difference in the frequency of heart defects is seen
• 8 of 14 embryos show hypertrophy of tricuspid valves
• co-treatment with PD0325901 and an inhibitor of histone H3K27 demethylase UTX, GSK-J4, ameliorates enlarged tricuspid valves, however, no difference in the frequency of heart defects is seen
• ventricular radius and the thickness of the tricuspid valves are higher
• 2 of 14 embryos exhibit ventricular septal defect
• increase in heart size at E16.5
• hypertrophy in pulmonary valve leaflets is prominent, plugging the entire space of the pulmonary valve ring
• E12.5 hearts show an enlarged pulmonary valve
• co-treatment with PD0325901 and an inhibitor of histone H3K27 demethylase UTX, GSK-J4, ameliorates enlarged pulmonary valves, however, no difference in the frequency of heart defects is seen
• 7 of 14 embryos show hypertrophy of pulmonary valves
• ventricular radius and the thickness of the pulmonary valves are higher
• 2 of 14 embryos show epicardial blisters
• at E16.5, about 9.8% of embryos are hemorrhagic and edematous
• about 11.1% of fetuses at E18.5 and E19.5 show alveolar hemorrhage
• however, lungs are able to inflate

immune system
• cavities such as the jugular lymphatic sacs from E12.5 to E14.5 are observed unlike in wild-type where they are hardly observed at this time, suggesting defective lymphatic development from the cardinal vein
• marker analysis indicates that embryos show defective lymphatic development from the cardinal vein, leading to distended and blood-filled jugular lymphatic sacs at E12.5 and E16.5, dilated lymphatic vessels and edema

craniofacial
• 5.1% of embryos show mandibular hypoplasia and kyphosis

homeostasis/metabolism
• embryos delivered by cesarean section at E18.5 and E19.5 are pale, without movement and gasp for breath with cyanotic appearance and death within a few hours
• at E16.5, about 9.8% of embryos are hemorrhagic and edematous

liver/biliary system
• decrease in liver weight is seen already at E16.5
• 88% of E18.5 fetuses show severe peripheral liver necrosis with decreased liver size and liver weight

mortality/aging
• no surviving mice are seen at weaning and survival rate of embryos drops after E16.5 indicating embryonic and neonatal lethality
• no surviving mice are seen at weaning and survival rate of embryos drops after E16.5 indicating embryonic and neonatal lethality
• treatment of pregnant mice with the MEK inhibitor PD0325901 partly rescues embryonic lethality and combined treatment with PD0325901 and a histone demethylase inhibitor further increases survival rate

muscle
• E14.5 hearts show an increase in density of trabeculae (hypertrabeculation)
• 4 of 14 embryos exhibit a thickened trabecular layer and thinned compact layer in the left, right, or combined myocardium
• 4 of 14 embryos exhibit a thickened trabecular layer and thinned compact layer in the left, right, or combined myocardium

respiratory system
• about 11.1% of fetuses at E18.5 and E19.5 show alveolar hemorrhage
• however, lungs are able to inflate

skeleton
• 5.1% of embryos show mandibular hypoplasia and kyphosis
• 5.1% of embryos show mandibular hypoplasia and kyphosis

growth/size/body
• increase in heart size at E16.5

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
cardiofaciocutaneous syndrome DOID:0060233 OMIM:PS115150
J:216228




Genotype
MGI:6164160
cn3
Allelic
Composition
Braftm1Tumg/Braftm1Tumg
Tg(CAG-cre)2Osb/0
Genetic
Background
involves: C57BL * C57BL/6J * DBA * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Tumg mutation (0 available); any Braf mutation (60 available)
Tg(CAG-cre)2Osb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• all mice die within 19 days of birth




Genotype
MGI:6164161
cn4
Allelic
Composition
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
Genetic
Background
involves: C57BL * C57BL/6J * DBA * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Tumg mutation (0 available); any Braf mutation (60 available)
Tg(CAG-cre)2Osb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• about 50% of mice survive for over 4 weeks, while 31% survive for over 74 weeks

growth/size/body
• heart weight to body weight ratio is higher
• however heart size at 26 weeks of age is normal, no differences in cardiomyocyte size or number and no fibrosis is seen
• 67% of females develop a unilateral ovarian cyst filled with serous fluid at 10 weeks of age
• at 26 weeks of age, 100% of females have unilateral or bilateral severe ovarian cysts
• although body weights of mice are normal from birth to 2 days, after 1 week, weights are lower

cardiovascular system
• cardiomyocytes from 2 of 4 mice exhibit irregularly shaped and heterogeneous size of mitochondria, while the other half of remaining mice have numerous granules in the mitochondria, indicating cell injury
• however, cardiomyocytes exhibit normal sarcomeric structure and sarcoplasmic reticulum
• 4 of 7 E16.5 mutants have a dramatic increase in the density of trabeculae
• thickened at E16.5
• echocardiography shows blood flow from the left atrium to right atrium in 3 of 7 adult mice, indicating presence of atrial septal defects
• heart weight to body weight ratio is higher
• however heart size at 26 weeks of age is normal, no differences in cardiomyocyte size or number and no fibrosis is seen
• 4 of 7 E16.5 mutants show thickened pulmonary valve leaflets
• pulsed-wave Doppler waveforms at the pulmonary valve show higher ejection speed, indicating pulmonary stenosis in adults
• pulmonary valve stenosis is induced by thickened valve leaflets without evidence of infundibular stenosis
• however, M-mode echocardiography shows normal systolic and diastolic function in adults

craniofacial
• skull lengths are shorter in 8 week old mice
• however, skull widths are normal

behavior/neurological
• in the contextual fear-conditioning test, mice show reduced freezing behavior in response to re-presentation of the context, indicating a learning deficit
• stomach milk contents are decreased in all mice, suggesting a feeding problem
• slight decrease in the moving speed in the open-field test and in the Y-maze test
• however, overall behavior of the mice is essentially normal in the open-field, light-dark transition test, and Y-maze
• slight decrease in the total rearing number in the open-field test

endocrine/exocrine glands
• 67% of females develop a unilateral ovarian cyst filled with serous fluid at 10 weeks of age
• at 26 weeks of age, 100% of females have unilateral or bilateral severe ovarian cysts

integument
• 68% of mice exhibit long and/or dystrophic nails
• 68% of mice exhibit long and/or dystrophic nails

limbs/digits/tail
• 50% of mice exhibit extra digits on the forelimbs, while 3.6% of mice exhibit extra digits on the hindlimbs

muscle
• cardiomyocytes from 2 of 4 mice exhibit irregularly shaped and heterogeneous size of mitochondria, while the other half of remaining mice have numerous granules in the mitochondria, indicating cell injury
• however, cardiomyocytes exhibit normal sarcomeric structure and sarcoplasmic reticulum
• 4 of 7 E16.5 mutants have a dramatic increase in the density of trabeculae

reproductive system
• 67% of females develop a unilateral ovarian cyst filled with serous fluid at 10 weeks of age
• at 26 weeks of age, 100% of females have unilateral or bilateral severe ovarian cysts
• 50% of females are infertile

skeleton
• skull lengths are shorter in 8 week old mice
• however, skull widths are normal
• some mice have hunched appearance such as kyphosis

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
cardiofaciocutaneous syndrome DOID:0060233 OMIM:PS115150
J:226977





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory