About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Braftm1Tumg
targeted mutation 1, Yoko Aoki
MGI:5902220
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
involves: BALB/c * C57BL * C57BL/6J * DBA MGI:6164158
cn2
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
involves: C57BL * C57BL/6J * DBA MGI:5902221
cn3
Braftm1Tumg/Braftm1Tumg
Tg(CAG-cre)2Osb/0
involves: C57BL * C57BL/6J * DBA * ICR MGI:6164160
cn4
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
involves: C57BL * C57BL/6J * DBA * ICR MGI:6164161


Genotype
MGI:6164158
cn1
Allelic
Composition
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
Genetic
Background
involves: BALB/c * C57BL * C57BL/6J * DBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Tumg mutation (0 available); any Braf mutation (60 available)
Tg(CAG-cre)2Osb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• a few mice survive to adulthood, although all mice die within 24 weeks
• about 50% of mice die at P5 and 90% at weaning (P28)

growth/size/body
• mice exhibit higher heart weight to body weight ratio at P5, indicating cardiomegaly

cardiovascular system
• 5 of 6 mice exhibit a large secundum atrial septal defect (ASDII), while 1 of 6 mice exhibit a small ASDII or patent foramen ovale
• however, cardiac valve defects and apparent abnormalities in other tissues are not seen
• 1 of 6 mice exhibit a small ASDII or patent foramen ovale
• mice exhibit higher heart weight to body weight ratio at P5, indicating cardiomegaly

integument

liver/biliary system
• 36% of mice show liver necrosis at P3

reproductive system
• females are infertile
• however, males are fertile




Genotype
MGI:5902221
cn2
Allelic
Composition
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
Genetic
Background
involves: C57BL * C57BL/6J * DBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Tumg mutation (0 available); any Braf mutation (60 available)
Tg(CAG-cre)2Osb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• 93% of hearts at E16.5 show various defects
• 3 of 14 embryos show hypoplasia of the coronary arteries
• E14.5 hearts show an increase in density of trabeculae (hypertrabeculation)
• 4 of 14 embryos exhibit a thickened trabecular layer and thinned compact layer in the left, right, or combined myocardium
• 4 of 14 embryos exhibit a thickened trabecular layer and thinned compact layer in the left, right, or combined myocardium
• hearts exhibit enhanced cell proliferation
• at E13.5, the interventricular septum and myocardium show enhanced cell proliferation
• at E16.5, the pulmonary valves and the interventricular septum in fetuses with ventricular septal defect, show enhanced cell proliferation
• 2 of 14 embryos exhibit abnormal endocardial cushion
• 9 of 14 embryos show hypertrophy of mitral valves
• co-treatment with PD0325901 and an inhibitor of histone H3K27 demethylase UTX, GSK-J4, ameliorates enlarged mitral valves, however, no difference in the frequency of heart defects is seen
• 8 of 14 embryos show hypertrophy of tricuspid valves
• co-treatment with PD0325901 and an inhibitor of histone H3K27 demethylase UTX, GSK-J4, ameliorates enlarged tricuspid valves, however, no difference in the frequency of heart defects is seen
• ventricular radius and the thickness of the tricuspid valves are higher
• 2 of 14 embryos exhibit ventricular septal defect
• increase in heart size at E16.5
• hypertrophy in pulmonary valve leaflets is prominent, plugging the entire space of the pulmonary valve ring
• E12.5 hearts show an enlarged pulmonary valve
• co-treatment with PD0325901 and an inhibitor of histone H3K27 demethylase UTX, GSK-J4, ameliorates enlarged pulmonary valves, however, no difference in the frequency of heart defects is seen
• 7 of 14 embryos show hypertrophy of pulmonary valves
• ventricular radius and the thickness of the pulmonary valves are higher
• 2 of 14 embryos show epicardial blisters
• at E16.5, about 9.8% of embryos are hemorrhagic and edematous
• about 11.1% of fetuses at E18.5 and E19.5 show alveolar hemorrhage
• however, lungs are able to inflate

immune system
• cavities such as the jugular lymphatic sacs from E12.5 to E14.5 are observed unlike in wild-type where they are hardly observed at this time, suggesting defective lymphatic development from the cardinal vein
• marker analysis indicates that embryos show defective lymphatic development from the cardinal vein, leading to distended and blood-filled jugular lymphatic sacs at E12.5 and E16.5, dilated lymphatic vessels and edema

craniofacial
• 5.1% of embryos show mandibular hypoplasia and kyphosis

homeostasis/metabolism
• embryos delivered by cesarean section at E18.5 and E19.5 are pale, without movement and gasp for breath with cyanotic appearance and death within a few hours
• at E16.5, about 9.8% of embryos are hemorrhagic and edematous

liver/biliary system
• decrease in liver weight is seen already at E16.5
• 88% of E18.5 fetuses show severe peripheral liver necrosis with decreased liver size and liver weight

mortality/aging
• no surviving mice are seen at weaning and survival rate of embryos drops after E16.5 indicating embryonic and neonatal lethality
• no surviving mice are seen at weaning and survival rate of embryos drops after E16.5 indicating embryonic and neonatal lethality
• treatment of pregnant mice with the MEK inhibitor PD0325901 partly rescues embryonic lethality and combined treatment with PD0325901 and a histone demethylase inhibitor further increases survival rate

muscle
• E14.5 hearts show an increase in density of trabeculae (hypertrabeculation)
• 4 of 14 embryos exhibit a thickened trabecular layer and thinned compact layer in the left, right, or combined myocardium
• 4 of 14 embryos exhibit a thickened trabecular layer and thinned compact layer in the left, right, or combined myocardium

respiratory system
• about 11.1% of fetuses at E18.5 and E19.5 show alveolar hemorrhage
• however, lungs are able to inflate

skeleton
• 5.1% of embryos show mandibular hypoplasia and kyphosis
• 5.1% of embryos show mandibular hypoplasia and kyphosis

growth/size/body
• increase in heart size at E16.5

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
cardiofaciocutaneous syndrome DOID:0060233 OMIM:PS115150
J:216228




Genotype
MGI:6164160
cn3
Allelic
Composition
Braftm1Tumg/Braftm1Tumg
Tg(CAG-cre)2Osb/0
Genetic
Background
involves: C57BL * C57BL/6J * DBA * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Tumg mutation (0 available); any Braf mutation (60 available)
Tg(CAG-cre)2Osb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• all mice die within 19 days of birth




Genotype
MGI:6164161
cn4
Allelic
Composition
Braftm1Tumg/Braf+
Tg(CAG-cre)2Osb/0
Genetic
Background
involves: C57BL * C57BL/6J * DBA * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Tumg mutation (0 available); any Braf mutation (60 available)
Tg(CAG-cre)2Osb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• about 50% of mice survive for over 4 weeks, while 31% survive for over 74 weeks

growth/size/body
• heart weight to body weight ratio is higher
• however heart size at 26 weeks of age is normal, no differences in cardiomyocyte size or number and no fibrosis is seen
• 67% of females develop a unilateral ovarian cyst filled with serous fluid at 10 weeks of age
• at 26 weeks of age, 100% of females have unilateral or bilateral severe ovarian cysts
• although body weights of mice are normal from birth to 2 days, after 1 week, weights are lower

cardiovascular system
• cardiomyocytes from 2 of 4 mice exhibit irregularly shaped and heterogeneous size of mitochondria, while the other half of remaining mice have numerous granules in the mitochondria, indicating cell injury
• however, cardiomyocytes exhibit normal sarcomeric structure and sarcoplasmic reticulum
• 4 of 7 E16.5 mutants have a dramatic increase in the density of trabeculae
• thickened at E16.5
• echocardiography shows blood flow from the left atrium to right atrium in 3 of 7 adult mice, indicating presence of atrial septal defects
• heart weight to body weight ratio is higher
• however heart size at 26 weeks of age is normal, no differences in cardiomyocyte size or number and no fibrosis is seen
• 4 of 7 E16.5 mutants show thickened pulmonary valve leaflets
• pulsed-wave Doppler waveforms at the pulmonary valve show higher ejection speed, indicating pulmonary stenosis in adults
• pulmonary valve stenosis is induced by thickened valve leaflets without evidence of infundibular stenosis
• however, M-mode echocardiography shows normal systolic and diastolic function in adults

craniofacial
• skull lengths are shorter in 8 week old mice
• however, skull widths are normal

behavior/neurological
• in the contextual fear-conditioning test, mice show reduced freezing behavior in response to re-presentation of the context, indicating a learning deficit
• stomach milk contents are decreased in all mice, suggesting a feeding problem
• slight decrease in the moving speed in the open-field test and in the Y-maze test
• however, overall behavior of the mice is essentially normal in the open-field, light-dark transition test, and Y-maze
• slight decrease in the total rearing number in the open-field test

endocrine/exocrine glands
• 67% of females develop a unilateral ovarian cyst filled with serous fluid at 10 weeks of age
• at 26 weeks of age, 100% of females have unilateral or bilateral severe ovarian cysts

integument
• 68% of mice exhibit long and/or dystrophic nails
• 68% of mice exhibit long and/or dystrophic nails

limbs/digits/tail
• 50% of mice exhibit extra digits on the forelimbs, while 3.6% of mice exhibit extra digits on the hindlimbs

muscle
• cardiomyocytes from 2 of 4 mice exhibit irregularly shaped and heterogeneous size of mitochondria, while the other half of remaining mice have numerous granules in the mitochondria, indicating cell injury
• however, cardiomyocytes exhibit normal sarcomeric structure and sarcoplasmic reticulum
• 4 of 7 E16.5 mutants have a dramatic increase in the density of trabeculae

reproductive system
• 67% of females develop a unilateral ovarian cyst filled with serous fluid at 10 weeks of age
• at 26 weeks of age, 100% of females have unilateral or bilateral severe ovarian cysts
• 50% of females are infertile

skeleton
• skull lengths are shorter in 8 week old mice
• however, skull widths are normal
• some mice have hunched appearance such as kyphosis

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
cardiofaciocutaneous syndrome DOID:0060233 OMIM:PS115150
J:226977





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory