Analysis Tools
mortality/aging
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• mice are viable and overtly normal at birth but die within 1 day of birth
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growth/size/body
microcephaly
(
J:240522
)
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• mice delivered by Caesarean section at E18.5 exhibit microcephaly
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nervous system
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• double immunofluorescence analysis using antibodies against betaIII tubulin (a neuronal marker) and cleaved Caspase-3 revealed increased neuron apoptosis in the occipital region of neopallium at E18.5
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• a significant increase in the number of cleaved Caspase-3-positive cells is noted in the occipital region of neopallium at E18.5
• however, no apoptotic cells are detected in other brain regions
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• at E18.5, both transverse and longitudinal distances in the brain are significantly shorter than those in control brains
• however, cellular organization is relatively normal
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• at E18.5
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• at E18.5
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• at E18.5, the occipital region of neopallium is smaller than normal while the difference in the parietal region is less apparent
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cellular
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• double immunofluorescence analysis using antibodies against betaIII tubulin (a neuronal marker) and cleaved Caspase-3 revealed increased neuron apoptosis in the occipital region of neopallium at E18.5
|
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• a significant increase in the number of cleaved Caspase-3-positive cells is noted in the occipital region of neopallium at E18.5
• however, no apoptotic cells are detected in other brain regions
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